Supercritical carbon dioxide extraction of four medicinal mediterranean plantsČižmek, Lara (Avtor) Bavcon Kralj, Mojca (Avtor) Čož-Rakovac, Rozelindra (Avtor) Mazur, Dmitry M. (Avtor) Ul'yanovskii, Nikolai (Avtor) Likon, Marko (Avtor) Trebše, Polonca (Avtor) green solventschamomileyarrowSt. John's wortcurry plantsquare-wave voltammetrychromatographyWith everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.20212021-09-23 12:37:46Članek v reviji131188UDK: 543.544ISSN pri članku: 1420-3049DOI: 10.3390/ molecules26185697COBISS_ID: 77584387sl