Application of antiSMASH tool for identification and analysis of type II polyketide gene clustersGrujić, Vladimir (Avtor) Petković, Hrvoje (Mentor) Starcevic, Antonio (Komentor) ActinomycetesantiSMASHbiosynthetic gene clusterbiotechnologytype II polyketide synthaseActinomycetes are one of the most economically and biotechnologically valuable prokaryotes considering that they produce wide range of bioactive secondary metabolites, such as antibacterials, antitumor agents and immunosuppressive agents. Aromatic polyketides are produced by type II polyketide synthases (type II PKSs). In recent years, genome mining has seen broad applications in identifying and characterizing new compounds, and metabolic engineering approaches have been applied to generate new analogues. The secondary metabolite analysis shell-antiSMASH has assisted researchers in this search for new potential bioactive compounds. Using the antiSMASH, we minned and analyzed 34 Acintomycetes genomes which contained type II PKS identified. We identified potentially new metabolites encoded BGCs from 9 Actinomycetes strains, for which we propose to produce novel structures. We observed that for predicting the structure of metabolite encoded by analysed BGC, the most important roles have the enzymes involved in tailoring modifications, especially oxygenases. We have demonstrated that antiSMASH represents the reliable in silico method for the analysis of Type II PKS BGC in case when good quality of genomes are available. Wet laboratory experiments would have to be carried out to confirm in silico predictions, which were generated in the scope of this work[V. Grujić]20212021-06-18 07:15:02Magistrsko delo/naloga127647UDK: 602.3:579.873.7:602.1:681.5:604.4:615.33(043.2)VisID: 191254COBISS_ID: 67559427sl