Razvoj in validacija metod čiščenja laboratorijske steklovine za odstranjevanje vanilina v pogojih dobre proizvodne prakseBožič, Anže (Avtor) Ilaš, Janez (Mentor) Andrenšek, Samo (Komentor) vanillingood manufacturing practiceGCHPLCUV-VISCleaning of laboratory glassware is one of the most basic, but at the same time also one of the most important processes, that takes place in every analytical laboratory. Adequacy of cleaning and equipment, which is used in the cleaning process, therefore have to be checked on a regular basis. Because of that it is essential, that the used testing procedure is as easy and as fast as possible. The purpose of this thesis was to develop a routine procedure and analytical methods for determination of adequacy of cleaning. Because of the property of its crystals to form an even thin layer all over the surface of laboratory glassware, vanillin was chosen as a testing substance. GC, HPLC and UV-VIS spectrophotometry methods were developed for vanillin detection. To confirm the identity of vanillin, its GC-MS spectrum was recorded as well. All experimental work was done under good manufacturing practice conditions and followed all its guidelines. We tried to develop analytical procedures in such way, that the same solutions could be used in validation of all three analytical methods. Methanol was chosen as a solvent. All methods were successfully validated according to ICH guidelines. Due to the longer retention time of vanillin and higher baseline noise, GC method was found to be the worst of them. HPLC and UV-VIS methods gave comparable results. HPLC method was used to test the adequacy of our washing machine. We found out that the chosen sampling procedure with the dissolution of any residues of vanillin is suitable for testing the adequacy of washing machine performance and with that for testing the laboratory glassware cleaning on a daily basis.20202020-08-29 08:45:06Magistrsko delo/naloga118662VisID: 74828sl