In vitro characterization of bioactivities of rhizome and flower extracts of Japanese (Fallopia japonica) and Bohemian (Fallopia x bohemica) knotweedBergant, Tina (Avtor) Pogačnik, Lea (Mentor) Viktorova, Jitka (Komentor) antioxidant activityknotweedFallopiaplant extractscancercytotoxicitycell lineHeLaHepG2PaTuHEK 293Tα-amylasediabetes mellitusantimicrobial activityanalytical methodsHPLCCAAThe aim of this research was to compare the antioxidant, anticancer, antidiabetic and antimicrobial activities of Japanese (Fallopia japonica) and Bohemian (Fallopia x bohemica) knotweed rhizome and flower ethanol extracts. Regarding antioxidant activity, rhizome extracts possess the highest antioxidant activity values, while in the assay of cellular antioxidant activity (CAA) with HepG2 cell line, the highest antioxidant activitiy was determined in flowers of F. x bohemica. Since the knotweed extracts are already used in pharmaceutic industry, the possibility of toxicity is very low. The haemolytic assay proved safe the use of our samples towards normal blood cells; moreover, by means of resazurin assay we also checked the possible cytotoxicity on different tissue cell lines, namely three cancer cell lines (HeLa, HepG2 and PaTu) and one non-cancer cell line HEK 293T. We found that the PaTu cell line is the least sensitive towards all knotweed extracts, whereas the HEK 293T cell line is the most sensitive. Flower extracts of both species are less toxic towards cells than the rhizomes ones. The extracts were also able to decrease α-amylase activity in an antidiabetic assay, the rhizome extracts being more effective than the flower ones. Regarding antimicrobial activity, the concentrated ethanol extracts are not able to inhibit bacterial growth; however, they are inhibiting the yeast growth, the rhizome extracts being more effective than the flower ones. We believe that the flowers and rhizomes of knotweeds indicate a new source of antioxidants, which could be used for prevention of diabetes and cancer.[T. Bergant]20192019-06-23 07:46:08Magistrsko delo/naloga108286UDK: 577.1:547.9:579.24:582.665VisID: 163892COBISS_ID: 5074808sl