Comprehensive analysis of 66 complete molluscum contagiosum virus (MOCV) genomesZorec, Tomaž Mark (Avtor) Alm, Erik (Avtor) Lind Karlberg, Maria (Avtor) Advani, Reza (Avtor) Hošnjak, Lea (Avtor) Poljak, Mario (Avtor) complete genome restriction mappingcomplete genome sequencingde novo assemblygenome annotationgenomic diversitymolluscipoxvirus harmonizationmolluscum contagiosum virusmolluscum contagiosum virus genotypingrecombinationFour molluscum contagiosum virus (MOCV) genotypes (MOCV1–4) and four subtype variants (MOCV1p, MOCV1va, MOCV1vb, and MOCV1vc) were partially characterized using restriction enzyme profiling in the early 1980s/1990s. However, complete genome sequences of only MOCV1 and MOCV2 are available. The evolutionary pathways of MOCV genotypes and subtype variants with unavailable sequences remain unclear, and also whether all MOCV genotypes/subtype variants can be reliably detected and appropriately categorized using available PCR-based protocols. We de novo fully characterized and functionally annotated 47 complete MOCV genomes, including two putative non-MOCV1/2 isolates, expanding the number of fully characterized MOCV genomes to 66. To ascertain the placement of any putative novel MOCV sequence into the restriction profiling typing scheme, we developed an original framework for extracting complete MOCV genome sequence-based restriction profiles and matching them with reference restriction profiles. We confirmed that two putative non-MOCV1/2 isolates represent the first complete genomes of MOCV3. Comprehensive phylogenomic, recombination, and restriction enzyme recognition site analysis of all 66 currently available MOCV genomes showed that they can be agglomerated into six phylogenetic subgroups (PG1–6), corresponding to the subtype variants from the pioneering studies. PG5 was a novel subtype variant of MOCV2, but no PGs corresponded to the subtype variants MOCV1vb or MOCV4. We showed that the phylogenetic subgroups may have diverged from the prototype MOCV genotype lineages following large-scale recombination events and hinted at partial sequence content of MOCV4 and direction of recombinant transfer in the events that spawned PG5 and the yet undetected subtype variant MOCV1vb.20232024-11-06 11:30:30Članek v reviji164649sl