Involvement of purinergic signaling components in selected structures of the forebrain of rats suffering from experimental autoimmune encephalomyelitisDokmanović, Tamara (Avtor) Kreft, Marko (Mentor) Dragić, Milorad (Komentor) multiple sclerosisexperimental autoimmune encephalomyelitiseN/CD73adenosine receptorsadenosine deaminaseAMPaseADPaseMultiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) accompanied by demyelination and subsequent neuronal damage that leads to permanent disability in young adults. The most commonly used experimental model for studying MS is experimental autoimmune encephalomyelitis (EAE), which exhibits main pathological features of the human disease. EAE is considered an inflammatory disease, and the neuroinflammation caused by EAE is tightly regulated by purinergic signaling. Various pathological events lead to neuronal damage, triggering release of large amounts of ATP, which then acts differently on purinoreceptors. Furthermore, exogenous ATP is removed by cascade hydrolysis by the action of the ectonucleotidase enzyme chain. The last step in ATP degradation is the dephosphorylation of AMP into adenosine by the action of ecto-5'-nucleotidase (eN/CD73). In this paper, we compare the expression levels of purinoreceptors A1R, A2AR, A2BR, and A3R, and the activity of purinergic enzymes, ecto-5'-nucleotidase, adenosine deaminase (ADA), AMPase and ADPase in selected brain regions of healthy control animals and animals in the peak of EAE, aiming to better understand the role of purinergic signaling in the pathophysiology of MS.[T. Dokmanović]20242024-10-10 08:15:44Magistrsko delo/naloga163672sl