Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayersDrabik, Dominik (Avtor) Drab, Mitja (Avtor) Penič, Samo (Avtor) Iglič, Aleš (Avtor) Czogalla, Aleksander (Avtor) lipid bilayersmicrodomainsMonte Carlo simulationsceramide 1 phosphateBiological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present signifcant challenges. In this study we employ spotvariation z-scan fuorescence correlation spectroscopy (svzFCS) tailored for artifcial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of defnitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of difusion coefcient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confrming that nano- and microdomains can co-exist in lipid vesicles.20232024-04-02 13:22:57Članek v reviji155446sl