Comparison of ADVIA Centaur ultra-sensitive and high-sensitive assays for troponin I in serumOsredkar, Joško (Avtor) Fabjan, Teja (Avtor) Kumer, Kristina (Avtor) Tršan, Jure (Avtor) Poljančič, Laura (Avtor) Košir, Miha (Avtor) Vovk, Pia (Avtor) Snoj, Nada (Avtor) Finderle, Petra (Avtor) Možina, Hugon (Avtor) acute coronary syndromemyocardial infarctiontroponin IverificationCardiac troponin I (cTnI) is a standard biomarker for the diagnosis of acute myocardial infarction (AMI). While older, ultra-sensitive cTnI (us-cTnI) assays use the 99th percentile as the reference threshold, newer high-sensitive cTnI (hs-cTnI) assays use the limit of detection or functional sensitivity instead. However, little has been done to systematically compare these two methods. The present study also served as a validation of hs-cTnI in our laboratory. Here, we compared the results obtained from the blood serum obtained from 8810 patients using the us-cTnI and the hs-cTnI assays run in tandem on the ADVIA Centaur XP analyser. We found that in 2279 samples the concentration of cTnI measured with the ultra-sensitive method was below the detection limit, while with the high-sensitive method, only 540 were below the detection limit. We also compared results from these assays with the ultimate diagnosis of a subset of individuals. The analysis of the results below cut-off with the ultra-sensitive method showed that this method would not detect 96 cases related to heart disorder. Overall, the main finding of our research is that hs-cTnI is the preferable option and is able to be deployed effectively in the laboratory setting.20222022-09-06 09:48:31Članek v reviji139678sl