Fermentation of CHO cells for biomass component quantificationKnez, Špela (Avtor) Narat, Mojca (Mentor) Borth, Nicole (Komentor) BiotechnologybioprocessingCHOmodelingbiomassChinese Hamster Ovary (CHO) cells are the main production host for recombinant therapeutic proteins. They have desirable process performance characteristics such as high expression rates, rapid growth and required post- translational modification. To further exploit its potential, genome scale metabolic model was made for CHO-K1 cell line. Since model is lacking correct biomass values of CHO-K1, which could improve model predictions, focus of this study was to determine biomass and biomass components during a batch process in exponential phase for producing and non producing cell line, grown with and without 8 mM Glutamine. For that, bioprocesses of each condition were performed and sampled 3 times per day to obtain data for growth characteristics, extracellular metabolites and to determine biomass composition. Biomass equation and biomas formula were calculated for each day and condition. Data show cell dry mass varies between strains, yet differences are not significant. Biomass compositional analysis revealed only small differences in amino acid composition between different CHO cell lines, but the composition changes over the time course. As the macromolecular composition changes over the batch, so must the energy requirements to synthesize these macromolecules. While results presented in this thesis may show a need to determine only proteins content for each cell line to improve cell specific predictions, further studies as energy maintenance and uptake/secretion rates are still needed to improve model predictions.[Š. Knez]20192019-09-07 07:45:53Magistrsko delo/naloga109719sl