Analiza krvne slike in proliferacija krvnih celic v kulturi pri proteusuPrša, Patrik (Avtor) Bizjak Mali, Lilijana (Mentor) Sessions, Stanley K. (Komentor) bloodhematologycell culturetissue explantsEuropean blind cave salamanderProteus anguinusThe purpose of this study was to develop non-destructive approaches to study cell biology, immunology and physiology in the European blind cave salamander (Proteus anguinus). Because of certain unique biological characteristics and its subterranean habitat in permeable limestone, the proteus is thought to be extremely vulnerable to different pollutants and to various potential pathogens that are of global concern to amphibians. Thus, developing non-destructive in vitro systems for research on the proteus is potentially an important approach in the conservation biology of this charizmatic species. Cell cultures in particular could provide an important source of material for a wide range of studies and can be done without sacrificing the animal or disrupting populations. We used small samples of blood to perform hematological analyses, including differential blood cell counts and neutrophil/lymphocyte ratios (NLR). We found that blood cell counts and differential WBC (white blood cell) counts did not change significantly in captivity and no statistically significant differences were found in the NLR between recently captured animals and animals kept in captivity for different lengths of time. Performing the same hematological analyses in diseased specimens likewise revealed no consistent pattern. Comparison of same individuals during different lengths of captivity showed no significant changes. We also developed two non-destructive cell culture methods: blood cell cultures and primary tissue explants from tail fin. Although our attempts to optimize blood cell cultures were only partially successful, we were able to optimize the protocol for primary tissue explants. Our results allow us to describe the dynamics of in vitro cellular migration and cellular lifespan for the first time in the proteus.20182018-09-13 07:46:30Magistrsko delo/naloga103023sl