The thesis has confirmed transmission and steady genetic transformation Cas9, Hpt II and ZsGreen into the plant Nicotiana benthamiana, which is gaining on the importance as a model organism. The gene transformation was carried out with the aid of a bacterium Agrobacterium tumefaciens which helped us to co-cultivate the leafy parts and which enabled import of the chosen genes into a plant. Adventitious regeneration of the transgenetic offshoot was carried out on a solid nutrient medium Murashige and Skoog with added 0.1 mg l-1 auxin 1-Naphthaleneacetic acid (NAA), 1 mg l-1 cytokinin 6- Benzylaminopurine (BAP), 30 g l-1 sucrose, 150 mg l-1 timentin, 25 mg l-1 higromicin and 8 g l-1 agar. The success of the transformation has been checked with PCR multiplication and agarose gel electrophoresis in T0 generation. It has proven the success of the tranmission of the genes, however, the presence of a bacterium Agrobacterium tumefaciens has also been found in traces. Therefore, the decision was made to acclimatize all the plants where the desired genes were present despite the source genes. A steady transformation has been confirmed in the T1 generation, which helped to confirm the presence of the desired genes and their inheritance in the next generation. Molecular analysis was performed with the help of the KAPA3G Plant PCR kit, which enabled faster and better results as it significantly shortened the way from a plant sample to the result.
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