Antibodies are a subclass of biopharmaceuticals used to treat a number of conditions related with impaired immune system. A high degree of purity essentially contributes to safety and quality of these complex substances. Affinity chromatography resins play a vital role in antibody purification process. The development of new approaches in purifying these type of molecules influences on lowering the prices of biopharmaceuticals on the market. The first affinity resins were based on proteins that are found on the surface of certain species of bacteria (protein A, G and L). Due to the progresses in the field of recombinant technology, protein engineering, combinatorial chemistry and later computer modeling of organic compounds new affinity ligands with improved physical and chemical properties were developed. With the improvement of these properties we are able to influence on dynamic binding capacity of the affinity resins, which is one of the main parameters in industrial antibody purification process. Biopharmaceutical companies carefully choose the manufacturer of affinity chromatography columns, regarding to their product, the performance of the affinity resin and its price. Some of the well-known manufacturers are Thermo Fischer Scientific, GE Healthcare Life Sciences and Waters Corporation.
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