Histološke spremembe anastomoze vene porte po elektrokemoterapiji z bleomicinom pri prašičih

Stupan, Urban (avtor), Đokić, Mihajlo (mentor), Čemažar, Maja (komentor)

Elektrokemoterapija (EKT) se uporablja tudi za zdravljenje neresektabilnih malignomov trebušne votline. Namen te raziskave je bil pokazati, da je EKT anastomoze prašičje portalne vene varna in učinkovita metoda, da bi razširili indikacije za EKT lokalno napredovalega karcinoma trebušne slinavke. Raziskava je bila narejena na 12 prašičih, razdeljenih v 4 skupine. Opazovali smo histološke spremembe anastomoze portalne vene in nativne spodnje vene kave ter anastomoze lienalne vene. Anastomoza portalne vene in intaktna vena kava je bila v kontrolni skupini tretirana samo z elektroporacijo, prašiči so bili evtanazirani 28. dan po operaciji. V poskusnih skupinah pa je bila anastomoza portalne vene in intakna vena kava tretirana z EKT, prašiči so bili evtanazirani 7., 14. in 28. dan po operaciji. Anastomoza lienalne vene ni bila v nobeni skupini tretirana z elektroporacijo ali EKT, služila je kot kontrolna anastomoza. Med kontrolno skupino in skupinami, zdravljenimi z EKT, ni bilo ugotovljenih izrazitih razlik. Elektroporacija je povzročila nepovratne poškodbe gladkih mišičnih celic v tuniki medii. Te bi lahko bile posledica ozkega območja ireverzibilne elektroporacije v bližini elektrod ali tromboze vasa vasorum v tuniki eksterni. Na podlagi odsotnosti žilnih zapletov in podobnih histoloških sprememb pri kontrolnih venah lahko sklepamo, da EKT anastomoze portalne vene v modelu zdravih prašičev z ustrezno antikoagulantno zaščito varna in tehnično izvedljiva metoda.

• Jezik: Slovenski jezik
• Ključne besede: Elektrokemoterapija, anastomoza portalne vene, rak trebušne slinavke
• Vrsta gradiva: Doktorsko delo/naloga
• Organizacija: MF - Medicinska fakulteta
• Leto izida: 2026
• PID: 20.500.12556/RUL-189724
• Datum objave v RUL: 14.10.2026

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Histologic changes of portal vein anastomosis after electrochemotherapy with bleomycin in a porcine model

Izvleček

Electrochemotherapy (ECT) is also used for the treatment of unresectable malignancies of the abdominal cavity. The aim of this study was to demonstrate that ECT of a porcine portal vein anastomosis is a safe and effective method, in order to expand the indications for ECT in locally advanced pancreatic cancer. The study was conducted on 12 pigs, divided into 4 groups. Histological changes of the portal vein anastomosis, the native inferior vena cava, and the splenic vein anastomosis were observed. In the control group, the portal vein anastomosis and intact vena cava were treated with electroporation only, and the pigs were euthanized 28 days after surgery. In the experimental groups, the portal vein anastomosis and intact vena cava were treated with ECT, and the pigs were euthanized on days 7, 14, and 28 after surgery. The splenic vein anastomosis was not treated with either electroporation or ECT in any group and served as the control anastomosis. No significant differences were observed between the control group and the ECT-treated groups. Electroporation caused irreversible damage to smooth muscle cells in the tunica media, which could be the result of a narrow zone of irreversible electroporation that may occur near the electrodes, or thrombosis of the vasa vasorum in the tunica externa. Based on the absence of vascular complications and the similar histological changes in the control veins, it can be concluded that ECT of the portal vein anastomosis is a safe and technically feasible method in a model of healthy pigs with perioperative anticoagulation.

• Ključne besede: Electrochemothrapy, portal vein anastomosis, pancreatic cancer