An evolutionarily conserved role for CTNNB1/ β-CATENIN in regulating the development of the corpus callosum

Parichha, Arpan (avtor), Datta, Debarpita (avtor), Singh, Amrita (avtor), Talwar, Ishita (avtor), Yadav, Shreya (avtor), Miroševič, Špela (avtor), Žakelj, Nina (avtor), Gosar, David (avtor), Osredkar, Damjan (avtor)

The corpus callosum (CC) is a major nerve bundle that connects the two hemispheres of the brain. Dysgenesis of the CC is associated with neurodevelopmental disorders such as the CTNNB1 syndrome. We identified that five individuals carrying CTNNB1 mutations displayed CC deficits. To explore CTNNB1/β-CATENIN-dependent mechanisms that regulate CC midline crossing, we examined mice with Ctnnb1 gain-of-function (GOF) or loss-of-function (LOF) selectively targeted to the early embryonic central nervous system midline using an Lmx1aCre driver. We identify that the Lmx1a lineage contributes to midline cell populations known to regulate CC pathfinding: the glial wedge, the indusium griseum glia, and a population of midline glutamatergic neurons. We find that each of these structures are affected in both GOF and LOF embryos, resulting in a profound disruption of CC crossing and formation of Probst bundles. Thus, regulated β-CATENIN function in midline cell populations is critical for CC development and its dysregulation may underlie the CC deficits associated with CTNNB1 syndrome.

• Jezik: Angleški jezik
• Ključne besede: neurodevelopment, molecular neuroscience, CTNNB1 syndrome, corpus callosum development
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 10 str, e1-e4
• Številčenje: Vol. 28, iss. 9, art. 113335
• PID: 20.500.12556/RUL-187161
• UDK: 616.8
• ISSN pri članku: 2589-0042
• DOI: 10.1016/j.isci.2025.113335
• COBISS.SI-ID: 245893635
• Datum objave v RUL: 09.09.2026

Gradivo je del revije

• Naslov: iScience
• Založnik: Cell Press, Elsevier
• ISSN: 2589-0042
• COBISS.SI-ID: 24098568

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.