Preventing moisture condensation on Petri dish lids

Kramer, Mateja (avtor), Ranogajec, Slaven (avtor), Panov, Pane (avtor), Begeš, Gaber (avtor), Hudoklin, Domen (avtor)

Moisture condensation on the lids of Petri dishes (PDs) during incubation is a common issue in microbiological laboratories, which affects microbiological water quality testing. This research focuses on the condensation problem during the use of Reasoner’s agar (R2A) in water testing for pharmaceutical applications. Condensation can distort microbial colony counts that potentially compromise the reliability of test results. The findings presented in this work are limited to the investigated incubation conditions and R2A agar systems. We conducted a detailed investigation into the factors contributing to condensation, particularly temperature gradients and relative humidity within vertically stacked PDs during incubation. Our experiments revealed that condensation is primarily driven by temperature differences between adjacent PDs, with higher condensation levels forming in dishes at the top of the stack. We explored various strategies, including improved air circulation and thermal insulation, but found that horizontal stacking of PDs effectively prevented condensation. These findings offer a viable method to enhance the accuracy of microbiological testing in pharmaceutical environments by mitigating moisture-related issues.

• Jezik: Angleški jezik
• Ključne besede: Petri dishes, condensation, microbiological water testing, reasoner’s agar, incubation conditions, temperature gradients
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2026
• Št. strani: 9 str.
• Številčenje: Vol. 37, no. 23, art. 235802
• PID: 20.500.12556/RUL-185006
• UDK: 621.317:579.67:543.3
• ISSN pri članku: 0957-0233
• DOI: 10.1088/1361-6501/ae74ff
• COBISS.SI-ID: 285431811
• Datum objave v RUL: 20.07.2026

Gradivo je del revije

• Naslov: Measurement science & technology
• Skrajšan naslov: Meas. sci. technol.
• Založnik: IOP Publishing
• ISSN: 0957-0233
• COBISS.SI-ID: 6000901

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Petrijevke, kondenzacija, mikrobiološko testiranje vode, Reasoner agar, inkubacijski pogoji, temperaturni gradienti