Vpliv spiruline (Limnospira platensis) in njenega ostanka po ekstrakciji fikocianina na rast micelija bukovega ostrigarja (Pleurotus ostreatus)

Čamernik Kramar, Matej (avtor), Gregori, Andrej (mentor)

V diplomski nalogi smo preučevali vpliv spiruline (Limnospira plantesis) in njenega ostanka po ekstrakciji fikocianina na rast micelija bukovega ostrigarja (Pleurotus ostreatus). Namen raziskave je bil opredeliti vpliv različnih deležev dodatka spiruline ali algnega ostanka na stopnjo rasti micelija ter njegovo gostoto v rastnem substratu, ki je bil v osnovi sestavljen iz bukove žagovine in pšeničnih otrobov. Pet poskusnih obravnavanj se je razlikovalo v količini dodane spiruline oz. algnega ostanka, poleg tega pa smo variirali tudi količino otrobov (10 in 20 % delež) in tako preverjali učinke dodatkov v različno hranljivih gojiščih. Gojitev smo izvedli v kozarcih in po 11. dnevu od inokulacije dnevno spremljali vraščanje micelija. Po zaključeni rasti micelija smo vizualno ocenili gostoto micelija. Rezultati so pokazali, da manjši dodatek spiruline rahlo pospeši stopnjo rasti micelija in poveča njegovo gostoto v rastnem substratu, medtem ko večji deleži delujejo zaviralno. To lahko pripišemo visoki hranilni vrednosti spiruline in njeni kompleksni celični strukturi, ki miceliju otežuje razgradnjo substrata. Algni ostanek, ki ima zaradi ekstrakcijskega procesa fragmentirane celične strukture in je nekoliko manj hranljiv, je v poskusih, v katerih smo dodali večji delež dodatka, povečal rast micelija in povečal gostoto micelija v substratu. Izsledki raziskave nakazujejo potencialno uporabo algnih dodatkov v rastni substrat za izboljšano rast micelija. Za boljše razumevanje presnovnih odnosov med algnimi surovinami in glivami so potrebne dodatne raziskave, ki bodo omogočile optimizacijo sestave substratov in določitev optimalnih ter mejnih deležev algnih dodatkov, tako za samo rast micelija, kot tudi za tvorbo trosnjakov.

• Jezik: Slovenski jezik
• Ključne besede: Bukov ostrigar, spirulina, fikocianin, algni ostanek, ekstrakcija fikocianina
• Vrsta gradiva: Diplomsko delo/naloga
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2026
• PID: 20.500.12556/RUL-182159
• Datum objave v RUL: 26.04.2026

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: The effect of spirulina (Limnospira platensis) and algal residue on the growth of oyster mushroom (Pleurotus ostreatus) mycelium

Izvleček

In the diploma thesis, we investigated the impact of Spirulina (Limnospira platensis) and its residue after phycocyanin extraction on the growth of beech oyster mushroom (Pleurotus ostreatus) mycelium. The aim of the study was to determine the influence of different proportions of added spirulina or algal residue on the mycelium growth rate and its density in the growth substrate, which was primarily composed of beech sawdust and wheat bran. Five experimental treatments differed in the amount of added spirulina or algal residue, and we also varied the amount of bran (10 % and 20 % proportions) to assess the effects of additives in substrates with different nutritional levels. Cultivation was conducted in jars, and we monitored mycelium colonization daily starting from the 11th day after inoculation. After the mycelium growth was complete, we visually assessed its density. The results showed that a smaller addition of spirulina slightly accelerates the mycelium growth rate and increases its density in the growth substrate, while larger proportions have an inhibitory effect. This can be attributed to the high nutritional value of spirulina and its complex cellular structure, which complicates substrate degradation by the mycelium. The algal residue, which has fragmented cellular structures and is slightly less nutritious due to the extraction process, increased mycelium growth and density in the substrate in treatments with higher additive proportions. The findings of the study suggest the potential use of algal additives in growth substrates for improved mycelium growth. Further research is needed to better understand the metabolic relationship between algal raw materials and fungi, enabling the optimization of substrate composition and the determination of optimal and limiting proportions of algal additives, both for mycelium growth and for fruiting body formation.

• Ključne besede: Oyster mushroom, spirulina, phycoycanin, algal residue, phycocyanin extraction