Pathogenic bacteria Campylobacter jejuni have developed adaptive mechanisms that enable them to survive in unfavorable environmental conditions and contribute to their resistance to adverse factors present in the food chain. Pseudomonas fragi are spoilage bacteria known for their ability to form biofilms, including multispecies biofilms with pathogenic bacteria such as bacteria C. jejuni. The formation of multispecies biofilms represents a key survival strategy for bacteria C. jejuni, which on its own forms biofilms poorly. In this master’s thesis, we investigated the interactions between bacteria C. jejuni and P. fragi under various temperatures, atmospheric conditions, and time points. Biofilm was evaluated by determining the cultivable cells and by measuring biofilm biomass using the crystal violet staining method. The results showed that bacteria P. fragi formed stable biofilms under all tested conditions, while bacteria C. jejuni showed improved survival in multispecies biofilms. The findings confirm that multispecies biofilm formation represents an important survival strategy for bacteria C. jejuni and contributes to its persistence in the food chain.
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