One-pot HPLC method for simultaneous quantification of hydrogen peroxide and organic peroxides

Gabrič, Alen (avtor), Hodnik, Žiga (avtor), Ilaš, Janez (avtor), Pajk, Stane (avtor)

Hydrogen peroxide and organic peroxides are widely used in the pharmaceutical and food industries as disinfectants, preservatives, and bleaching agents. They can also form as by-products during polymerization, drying, or degradation processes, potentially compromising product quality. While current analytical methods typically measure total peroxide content, they do not distinguish between hydrogen peroxide and organic peroxides, despite their differing reactivity and implications for product stability. We developed a one-pot HPLC method for the simultaneous quantification of hydrogen peroxide and organic peroxides. The method utilizes borinic acid for the selective reaction with hydrogen peroxide, followed by the reaction of triphenylphosphine with the remaining organic peroxides. The resulting phenol derivative and triphenylphosphine oxide are detected using HPLC with UV detection. The method demonstrated strong performance across a variety of complex matrices, including pharmaceutical excipients, milk, honey, and vegetable oil. Quantification limits were 4.4 nmol/g for hydrogen peroxide and 9 nmol/g for organic peroxides. The method's practical utility was demonstrated using famotidine, a pharmaceutical compound selectively oxidized by hydrogen peroxide but not by organic peroxides. This application highlights the method's ability to guide excipient selection based on specific peroxide profiles and to support antioxidant use in formulation development. By enabling the differentiation of peroxide species, this approach facilitates the development of more stable and cost-effective formulations. This novel HPLC method offers a simple, robust, and versatile tool for the selective quantification of hydrogen peroxide and organic peroxides in a wide range of applications, including pharmaceutical and food analysis, where precise peroxide monitoring is essential.

• Jezik: Angleški jezik
• Ključne besede: peroxide quantification, hydrogen peroxide, organic peroxides, borinic acid, liquid chromatography, HPLC
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 8 str.
• Številčenje: Vol. 217, art. 114889
• PID: 20.500.12556/RUL-175790
• UDK: 543.426
• ISSN pri članku: 1095-9149
• DOI: 10.1016/j.microc.2025.114889
• COBISS.SI-ID: 255033859
• Datum objave v RUL: 07.11.2025

Gradivo je del revije

• Naslov: Microchemical journal
• Založnik: Elsevier
• ISSN: 1095-9149
• COBISS.SI-ID: 23088645

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kvantifikacija peroksida, vodikov peroksid, organski peroksidi, borova kislina, tekočinska kromatografija, HPLC, analizna kemija