In this work, we present the results of experiments conducted using a variety of tools for the segmentation of microscopic images. In the first part, we describe the tomography process, explaining how it works and the steps necessary for the construction of tomographic images. We provide a detailed description of the test data and the tools used. The testing procedure and the environment in which the experiments were conducted are also described, including the specific settings and adjustments necessary for the functioning of each tool. In the second part of the work, we present the results of our experiments, supported by visual material. We analyze the effectiveness and accuracy of each tool and discuss the respective advantages and disadvantages of each approach. Special attention is given to the challenges associated with using dated tools that rely on older libraries and methods as well as their limitations.
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