Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations

Batista Napotnik, Tina (avtor), Cimperman, Tina (avtor), Rems, Lea (avtor)

Electroporation affects action potential generation in excitable cells such as nerve, muscle and cardiac cells. Since electroporation-based treatments use different pulse protocols, we investigated how electric pulses of different duration (from 500 ns to 1 ms) trigger action potentials and cause electroporation in genetically engineered cultured excitable cell model. Transmembrane voltage was monitored using a fluorescent potentiometric probe and fluorescence microscopy. We triggered similar responses in these cells using electric pulses of all pulse durations. At lower electric fields, we stimulated action potentials and at higher electric fields, electroporation occurred: the action potentials were gradually prolonged and eventually, ended up in sustained depolarization. For shorter pulses, higher electric fields had to be used to achieve the same effect. However, the shape of the action potential was affected by pulse duration. With shorter pulses (500 ns–10 µs), the action potentials were much longer already at the excitation thresholds, due to more prominent effect of electroporation, compared to longer pulses (e.g., 1 ms) where action potentials were triggered by classical electrostimulation (i.e., excitation) without electroporation. Moreover, we detected a complex, biphasic intracellular calcium response in excitable S-HEK cells that was absent in nonexcitable NS-HEK version of these cells.

• Jezik: Angleški jezik
• Ključne besede: electroporation, exitable cells, action potential, pulse duration, intracellular calcium
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: 17 str.
• Številčenje: 15, art. 23451
• PID: 20.500.12556/RUL-170858
• UDK: 602.621
• ISSN pri članku: 2045-2322
• DOI: 10.1038/s41598-025-06989-5
• COBISS.SI-ID: 242427395
• Datum objave v RUL: 18.07.2025

Gradivo je del revije

• Naslov: Scientific reports
• Skrajšan naslov: Sci. rep.
• Založnik: Springer Nature
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: elektroporacija, vzdražne celice, akcijski potencial, dolžina pulza, znotrajcelični kalcij