Performance evaluation of a novel MBT LipidART module (Bruker Daltonics) for detection of colistin resistance in Escherichia coli and Klebsiella pneumoniae

Germ, Julija (avtor), Pirš, Mateja (avtor)

We evaluated the MBT LipidART module on a MALDI Biotyper® Sirius System (Bruker Daltonics) for the rapid detection of colistin resistance in Escherichia coli (EC) and Klebsiella pneumoniae (KPN) by analysing lipid A profiles in negative ion mode. Categorical agreement was achieved for 98.3% EC (N = 58) and 85.0% KPN (N = 40). Challenges included calibration difficulties, limited availability of compatible equipment and issues with mucoid and adherent KPN isolates that yielded invalid results. While MBT LipidART module shows promise as a rapid tool for detection of colistin resistance, its performance was notably better for EC compared to KPN.

• Jezik: Angleški jezik
• Ključne besede: performance evaluation, MBT LipidART module, detection of colistin resistance, Escherichia coli, Klebsiella pneumoniae
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.03 - Drugi znanstveni članki
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2025
• Št. strani: Str. 1501–1504
• Številčenje: Vol. 44, iss. 6
• PID: 20.500.12556/RUL-169431
• UDK: 616-097:616.1
• ISSN pri članku: 0934-9723
• DOI: 10.1007/s10096-025-05099-4
• COBISS.SI-ID: 230357763
• Datum objave v RUL: 28.05.2025

Gradivo je del revije

• Naslov: European journal of clinical microbiology & infectious diseases
• Skrajšan naslov: Eur. j. clin. microbiol. infect. dis.
• Založnik: Springer Nature
• ISSN: 0934-9723
• COBISS.SI-ID: 590356

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ocena učinkovitosti, modul MBT LipidART, odkrivanje odpornosti na kolistin, Escherichia coli, Klebsiella pneumoniae