Primerjava hiper- in normobaričnega kisika kot kontrastnega sredstva pri magnetnoresonančnem slikanju možganov

Velej, Vida (avtor), Cankar, Ksenija (mentor), Vidmar, Jernej (komentor)

Raztopljeni kisik ima znane paramagnetne učinke pri magnetnoresonančnem slikanju (MRI). Z raziskavo smo želeli primerjati učinke normobarične oksigenacije (NBO) in hiperbarične oksigenacije (HBO) na intenziteto signala MRI v človeških možganih. Prvi del: izhodiščno MRI možganov je bilo opravljeno na 17 zdravih preiskovnacih. Pri preiskovancih smo enak protokol MRI ponovili po izpostavljenosti NBO in HBO trikrat zaporedoma. Primerjali smo intenzitete signala različnih struktur na T1-, T2-poudarjenem slikanju ter pri slikanju z metodo izničenja tekočine (FLAIR) med preiskavami. Drugi del: izhodiščno MRI možganov je bilo opravljeno na 12 zdravih preiskovancih, enak protokol MRI smo ponovili po izpostavljenosti NBO in HBO trikrat zaporedoma, primerjali smo relaksacijska časa T1 in T2 v različnih strukturah. V beli in globoki sivi možganovini, likvorju, venski krvi in steklovini smo po izpostavitvi NBO in HBO opazili povečano intenziteto T1-poudarjenega signala brez pomembnih razlik med obema načinoma oksigenacije. Hiperoksigenacija je vplivala na intenziteto T2-poudarjenega signala le v posameznih strukturah brez pomembnih razlik med vplivoma HBO in NBO. Intenziteta signala FLAIR se je po hiperoksigenaciji spremenila le v steklovini in kavdatnem jedru; statistično značilne razlike med NBO in HBO pa so bile vidne v večini opazovanih struktur. NBO in HBO sta vplivala na relaksacijski čas T1 v številnih regijah bele in sive možganovine; različen vpliv NBO in HBO je bil viden le v nekaterih regijah. Relaksacijski čas T2 se je spremenil po NBO le v beli možganovini velikih možganov, po HBO pa poleg beline tudi v talamusu in nekaterih kortikalnih regijah; značilno razliko med vplivoma NBO in HBO na relaksacijski čas T2 smo opazili le v putamnu. Naši rezultati kažejo, da se pri MRI človeških možganov po izpostavljenosti NBO in HBO spremenijo intenzitete signalov na T1-poudarjenih slikah in slikah FLAIR; strukturam se po izpostavljenosti NBO in HBO spremeni relaksacijski čas T1, na relaksacijski čas T2 pa vpliva predvsem izpostavljenost HBO.

• Jezik: Slovenski jezik
• Ključne besede: magnetnoresonančno slikanje, možgani, hiperbarični kisik, normobarični kisik
• Vrsta gradiva: Doktorsko delo/naloga
• Organizacija: MF - Medicinska fakulteta
• Leto izida: 2025
• PID: 20.500.12556/RUL-169162
• Datum objave v RUL: 16.05.2025

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Comparison of Hyper- and Normobaric Oxygen as Contrast Agent in Magnetic Resonance Brain Imaging

Izvleček

Dissolved oxygen has known paramagnetic effects in magnetic resonance imaging (MRI). The aim of this study was to compare the effects of normobaric oxygenation (NBO) and hyperbaric oxygenation (HBO) on human brain MRI. First part: baseline brain MRI was performed in 17 healthy subjects, the same MRI protocol was repeated after exposure to NBO and HBO at three time points sequentially; signal intensities of several structures were measured on T1-weighted, T2-weighted and fluid attenuated inversion recovery (FLAIR) images. Second part: baseline brain MRI was performed in 12 healthy subjects, the same MRI protocol was repeated after exposure to NBO and HBO at three time points sequentially; T1- and T2-relaxation times of several intracranial structures were measured. Increased T1-weighted signal intensities were observed in white and deep grey brain matter, cerebrospinal fluid, venous blood and vitreous body after NBO and HBO compared to baseline without significant differences between both protocols. Hyperoxygenation affected T2-weighted signal intensities in few regions with no significant difference between HBO and NBO. Hyperoxygenation affected FLAIR signal intensities in vitreous body (NBO and HBO) and caudate nucleus (NBO); statistically significant differences in FLAIR signal intensities were found between NBO and HBO in most observed brain structures. NBO and HBO affected T1-relaxation time in various white and grey brain matter regions; significant differences in T1-relaxation time were found between NBO and HBO in some of them. T2-relaxation time was altered after NBO in cerebral white matter and after HBO in cerebral white matter, thalamus and some cortical regions; significant differences in T2-relaxation time between NBO and HBO were observed only in putamen. Our results show that in MRI of human brain, NBO and HBO change signal intensities on T1-weighted and FLAIR images; both NBO and HBO alter T1-relaxation time, while T2-relaxation time is affected predominantly by HBO.

• Ključne besede: magnetic resonance imaging, brain, hyperbaric oxygen, normobaric oxygen