Genomic revelations : investigating rotavirus a presence in wild ruminants and its zoonotic potential

Šenica Kavčič, Petra (avtor), Žele-Vengušt, Diana (avtor), Vengušt, Gorazd (avtor), Kuhar, Urška (avtor)

Introduction: Rotaviruses A (RVA) are a major cause of acute viral gastroenteritis in humans worldwide and are responsible for about two million hospitalizations per year. They can also infect other mammals such as pigs, calves, goats, lambs, and horses, in which they are also considered a major cause of viral diarrhea. While RVA is well studied in humans and domestic animals, its occurrence in wild ruminants is not well known. The RVA genome is a double-stranded RNA consisting of 11 segments, and genotyping is based on the VP7 (G) and VP4 (P) segments. Currently, there are 42G genotypes and 58P genotypes. RVA has a high mutation rate, and some combinations of G and P genotypes can infect different animal species, leading to speculation about the potential for zoonotic transmission. Materials and methods: A total of 432 fecal samples were collected from roe deer, red deer, chamois, mouflon and Alpine ibex in Slovenia between 2017 and 2021. To investigate the presence of RVA in wild ruminants, real-time RT-PCR was used. Positive samples were subjected to next generation sequencing (NGS) using RIP-seq method. Results and discussion: In total, 7 samples were RVA positive. Complete genomes were determined and phylogenetically analyzed for all 7 RVAs. Four different genotype constellations were present in 7 positive RVA animals: G8-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3, G6-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3, G10-P[15]-I2-R2-C2-M2-A3-N2-T6-E2-H3 and G10-P[15]-I2-R2-C2-M2-A11-N2-T6-E2-H3. Genotypes G6P[14] and G10P[15] were found in both roe deer and red deer, representing the first confirmed occurrence of RVA in red deer. In addition, genotype G8P[14] was found in chamois, representing the first known case of positive RVA in this species. Some of these genotypes have also been found in humans, indicating the potential for zoonotic transmission.

• Jezik: Angleški jezik
• Ključne besede: rotaviruses, wild ruminants, zoonotic potential, phylogenetic analysis, genomic diversity, cross-species infection, zoonoses, wild animals
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 9 str.
• Številčenje: Vol. 11, art. 1429654
• PID: 20.500.12556/RUL-166845
• UDK: 636.09:616
• ISSN pri članku: 2297-1769
• DOI: 10.3389/fvets.2024.1429654
• COBISS.SI-ID: 204664067
• Datum objave v RUL: 27.01.2025

Gradivo je del revije

• Naslov: Frontiers in veterinary science
• Skrajšan naslov: Front. vet. sci.
• Založnik: Frontiers Media S.A.
• ISSN: 2297-1769
• COBISS.SI-ID: 3969402

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Program financ.: Young researchers

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana