Primerjava učinkovitosti različnih tipov inokuluma za prenos viroida HSVd pri navadni kumari (Cucumis sativus)

Rozman, Maj (avtor), Jakše, Jernej (mentor), Volk, Helena (komentor)

Kumara (Cucumis sativus) je pomembna kmetijska rastlina, ki jo poleg drugih patogenov ogrožajo tudi razni viroidi, med njimi viroid zakrnelosti hmelja (HSVd). Okužba s HSVd povzroča deformacije, zakrnelo rast in zmanjšanje pridelka, kar predstavlja velik gospodarski problem. Namen magistrske naloge je bil primerjati učinkovitost različnih tipov inokuluma za prenos HSVd pri kumarah ter raziskati možne alternativne vire inokuluma. V okviru poskusa smo pripravili RNA-transkripte v pozitivni in negativni orientaciji, plazmide z zapisom za HSVd ter PCR-produkt z zapisom za HSVd. Dve sorti kumar, 'Suyo' in 'Marketer', smo inokulirali mehansko z različnimi tipi inokuluma, nato pa uspešnost okužbe preverili z metodo RT-PCR in agarozno gelsko elektroforezo. Po teh analizah smo prisotnost viroida potrdili še s sekvenciranjem. Inokulirali smo 50 rastlin vsake sorte. Rezultati so pokazali, da je bila najvišja stopnja okuženosti dosežena pri uporabi RNA-transkripta v pozitivni orientaciji, kjer smo pri obeh sortah dosegli 100 % okužbo. Pri inokulaciji s plazmidom je bila stopnja okuženosti 50 %, medtem ko je bila pri PCR-produktih PCR okuženost 50-% za sorto 'Suyo' in 40-% za sorto 'Marketer'. RNA-transkript v negativni orientaciji je povzročil 30-% okuženost pri obeh sortah.

• Jezik: Slovenski jezik
• Ključne besede: navadna kumara, viroid HSVd, viroid zakrnelosti hmelja, inokulacija, RT-PCR
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2024
• PID: 20.500.12556/RUL-166134
• COBISS.SI-ID: 220198915
• Datum objave v RUL: 21.12.2024

ROZMAN, Maj, 2024, Primerjava učinkovitosti različnih tipov inokuluma za prenos viroida HSVd pri navadni kumari (Cucumis sativus) [na spletu]. Magistrsko delo. [Dostopano 4 maj 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=166134

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Comparing the efficacy of different types of inoculum for HSVd transmission in common cucumber (Cucumis sativus)

Izvleček

Cucumber (Cucumis sativus) is an important agricultural plant that, in addition to other pathogens, is also threatened by various viroids, including hop stunt viroid (HSVd). Infection with HSVd causes deformities in plants, stunted growth and yield reduction, which is a major economic problem. The aim of this master's thesis was to compare the efficiency of different types of inoculum for the transmission of HSVd in cucumbers and to investigate possible alternative sources of inoculum. As part of the experiment, we prepared RNA transcripts in positive and negative orientation, a plasmid with HSVd transcript and a PCR product with HSVd transcript. Two cucumber varieties, 'Suyo' and 'Marketer', were then inoculated mechanically with different types of inoculum. The success of this process was checked by RT-PCR and agarose gel electrophoresis. After these analyses were concluded, the presence of the viroid was confirmed by sequencing. We inoculated 50 plants of each variety. The results showed that the highest level of infection was achieved when using the RNA-transcript in the positive orientation, where we achieved 100 % infection in both varieties. Plasmid inoculation had a 50 % infection rate, while PCR products had an infection rate of 50 % for 'Suyo' and 40 % for 'Marketer'. RNA-transcript in the negative orientation had 30 % infectivity in both varieties.

• Ključne besede: common cucumber, viroid HSVd hop stunt viroid, inoculation, RT-PCR