Analiza proteoma hemolimfe raka enakonožca Porcellio scaber po izpostavitvi mikroplastiki

Kukenberger, Karin (avtor), Leonardi, Adrijana (mentor), Jemec Kokalj, Anita (komentor)

Plastika je danes velik okoljski problem, saj njeni odpadki trajno onesnažujejo okolje. Poseben problem predstavlja mikroplastika (MP), majhni delci plastike (< 1 mm), ki nastajajo ob razgradnji večjih kosov in so biološko bolj dostopni. MP je prisotna v kopenskih in vodnih okoljih ter vpliva na organizme, vključno s kopenskimi raki enakonožci vrste Porcellio scaber, ki so bili predmet študije. Namen magistrskega dela je bil z uporabo proteomike preučiti morebitne spremembe v hemolimfi ob izpostavljenosti modelnega organizma MP. Opazovali smo učinek različnih vrst in koncentracij MP na izražanje proteinov v hemolimfi rakov P. scaber. Ugotovili smo, da izpostavljenost MP povzroča spremembe v izražanju proteinov, pri čemer so višje koncentracije MP povzročile izrazitejše spremembe. Raziskava je pokazala, da konvencionalna in biorazgradljiva mikroplastika obe povzročita spremembe v proteomu hemolimfe, kar zavrača hipotezo, da bi biorazgradljiva mikroplastika imela manjši vpliv. Ugotovljene spremembe v izražanju proteinov kažejo na stresni odziv organizmov na prisotnost MP v okolju, vključno z aktivacijo imunskega sistema in spremembami v metabolizmu. Študija potrjuje, da je MP resen okoljski dejavnik, ki vpliva na fiziološke procese pri organizmih, ter izpostavlja potrebo po nadaljnjih raziskavah njenega vpliva.

• Jezik: Slovenski jezik
• Ključne besede: mikroplastika, proteom, LDPE, PBAT, proteini
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Založnik: [K. Kukenberger]
• Leto izida: 2024
• PID: 20.500.12556/RUL-164295
• UDK: 591.113(043.2)
• COBISS.SI-ID: 212947203
• Datum objave v RUL: 20.10.2024

KUKENBERGER, Karin, 2024, Analiza proteoma hemolimfe raka enakonožca Porcellio scaber po izpostavitvi mikroplastiki [na spletu]. Magistrsko delo. K. Kukenberger. [Dostopano 21 april 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=164295

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Proteome analysis of isopod Porcellio scaber haemolymph after microplastics exposure

Izvleček

Plastics, which were developed in the 19th century, have become a major environmental problem today, as their waste permanently pollutes the environment. One particular problem is microplastics (MP), small plastic particles that are formed when larger parts decompose and are more biologically accessible. MP occurs in both terrestrial and aquatic environments and affects organisms, including terrestrial isopods of the species Porcellio scaber, which were the subject of this study. The aim of the MSc thesis was to use proteomics to investigate potential changes in the hemolymph when the model organism is exposed to MP. The response to different types and concentrations of MP on protein expression in the hemolymph of the isopod P. scaber was observed. It was found that exposure to MP leads to changes in protein expression, with higher concentrations of MP leading to more pronounced changes. The study showed that conventional and biodegradable plastics cause similar changes in the hemolymph proteome, disproving the hypothesis that biodegradable plastics would have a lesser effect. The observed changes in protein expression indicate a stress response of the organisms to the presence of MP, including activation of the immune system and changes in metabolism. The study confirms that MP is a serious environmental factor affecting physiological processes in organisms and emphasises the need for further research of its effects.

• Ključne besede: mikroplastic, proteom, LDPE, PBAT, protein