The mysteries of DNA preservation in bone : a comparative study of petrous bones and metacarpal epiphyses using ATR-FTIR spectroscopy

Leskovar, Tamara (avtor), Jerman, Ivan (avtor), Zupanič-Pajnič, Irena (avtor)

A comparative analysis of 26 petrous bones and epiphyses of metacarpals from the Second World War era revealed no significant differences in DNA yield or success in STR typing. This unexpected parity in DNA preservation between the petrous bone, a renowned source of endogenous DNA in skeletal remains, and the epiphyses of metacarpals, which are porous and susceptible to taphonomic changes, is surprising. In this study, we introduced ATR-FTIR spectroscopy as an approach to unravel the correlation between bone molecular structure and DNA preservation. Metacarpals and petrous bones with same taphonomic history were sampled and prepared for DNA analyses. While one portion of the sample was used for DNA analysis, the other underwent ATR-FTIR spectroscopic examination. The normalized spectra and FTIR indices between the epiphyses of metacarpals and petrous bones were compared. Because the taphonomic history of the remains used is relatively short and stable, the ATR-FTIR spectroscopy unveiled subtle structural differences between the two bone types. Petrous bones exhibited higher mineralization, whereas epiphyses contained more organic matter. The unexpected preservation of DNA in the epiphyses of metacarpals can likely be attributed to the presence of soft tissue remnants within the trabeculae. Here observed differences in the molecular structure of bones indicate there are different mechanisms enabling DNA preservation in skeletal tissues.

• Jezik: Angleški jezik
• Ključne besede: ATR-FTIR spectroscopy, DNA preservation, metacarpals, molecular structure, petrous bone, soft tissue remnants
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FF - Filozofska fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 8 str.
• Številčenje: Vol. 360, art. 112076
• PID: 20.500.12556/RUL-158505
• UDK: 61
• ISSN pri članku: 1872-6283
• DOI: 10.1016/j.forsciint.2024.112076
• COBISS.SI-ID: 198838019
• Datum objave v RUL: 14.06.2024

Gradivo je del revije

• Naslov: Forensic science international
• Založnik: Elsevier
• ISSN: 1872-6283
• COBISS.SI-ID: 23017945

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ATR-FTIR spektroskopija, ohranjanje DNK, metakarpalne kosti, molekularna struktura, petrorozna kost, ostanki mehkih tkiv

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-3080
• Naslov: Določitev predniškega porekla iz DNA za identifikacijske namene

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P2-0393
• Naslov: Napredni materiali za nizkoogljično in trajnostno družbo