Effects of electroporation on the function of sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase and ▫$Na^+$▫,▫$K^+$▫-ATPase in H9c2 cells

Jan, Vid (avtor), Jusović, Maida (avtor), Miklavčič, Damijan (avtor)

Pulsed field ablation (PFA) is a promising new treatment for atrial fibrillation (AF), in which pulmonary vein isolation is achieved by irreversible electroporation. Electroporation causes ATP to leak through the permeabilized membrane. ATP is required both for the healing of the cell membrane and for the functioning of ion pumps, such as sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase (SERCA) or ▫$Na^+$▫,▫$K^+$▫-ATPase (NKA), which play a key role in maintaining continuous contractions of the heart muscle. We investigated the effects of electroporation on the expression of ion pumps and possible correlations with the activation of AMPK, the main energy sensor in cells. H9c2 rat cardiac cells were exposed to either monopolar or bipolar (H-FIRE) pulses. Cells lysed 4 or 24 h after electroporation were used for mRNA and protein expression analyses. Overall, both pulse protocols caused a dosedependent downregulation of crucial SERCA and NKA isoforms, except for NKA▫$\alpha$▫2 and ▫$\beta$▫3, which were upregulated after 24 h. Monopolar pulses also decreased the phosphorylation of FXYD1, which may cause an inhibition of NKA activity. Both pulse protocols caused an increased AMPK activity, which may decrease both SERCA and NKA activity via calcium/calmodulin-dependent protein kinase. Our results provide important new insights into what happens in surviving cardiomyocytes after they are exposed to PFA.

• Jezik: Angleški jezik
• Ključne besede: irreversible electroporation, reversible electroporation, pulsed field ablation, sarco/endoplasmic reticulum Ca2+-ATPase, Na+, K+-ATPase, atrial fibrillation, AMPK activation, H-FIRE pulses, H9c2 cells
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 21 str.
• Številčenje: Vol. 14, iss. 7 [article no.] 2695
• PID: 20.500.12556/RUL-156319
• UDK: 602.621
• ISSN pri članku: 2076-3417
• DOI: 10.3390/app14072695
• COBISS.SI-ID: 190227203
• Datum objave v RUL: 20.05.2024

Gradivo je del revije

• Naslov: Applied sciences
• Skrajšan naslov: Appl. sci.
• Založnik: MDPI
• ISSN: 2076-3417
• COBISS.SI-ID: 522979353

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ireverzibilna ekektroporacija, reverzibilna elektroporacija, ablacija s pulznim električnim poljem, Ca2+-ATPaza sarko/endoplazemskega retikuluma, Na+, K+-ATPaza, atrijska fibrilacija, aktivacija AMPK, H-FIRE pulzi, celice H9c2

Projekti

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: P2-0249
• Naslov: Elektroporacija v biologiji, biotehnologiji in medicini

• Financer: ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
• Številka projekta: I0-0022
• Naslov: Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)