Cationised fibre-based cellulose multi-layer membranes for sterile and high-flow bacteria retention and inactivation

Kokol, Vanja (avtor), Kos, Monika (avtor), Vivod, Vera (avtor), Gunde-Cimerman, Nina (avtor)

Low-cost, readily available, or even disposable membranes in water purification or downstream biopharma processes are becoming attractive alternatives to expensive polymeric columns or filters. In this article, the potential of microfiltration membranes prepared from differently orientated viscose fibre slivers, infused with ultrafine quaternised (qCNF) and amino-hydrophobised (aCNF) cellulose nanofibrils, were investigated for capturing and deactivating the bacteria from water during vacuum filtration. The morphology and capturing mechanism of the single- and multi-layer structured membranes were evaluated using microscopic imaging and colloidal particles. They were assessed for antibacterial efficacy and the retention of selected bacterial species (Escherichia coli, Staphylococcus aureus, Micrococcus luteus), differing in the cell envelope structure, hydrodynamic biovolume (shape and size) and their clustering. The aCNF increased biocidal efficacy significantly when compared to qCNF-integrated membrane, although the latter retained bacteria equally effectively by a thicker multi-layer structured membrane. The retention of bacterial cells occurred through electrostatic and hydrophobic interactions, as well as via interfibrous pore diffusion, depending on their physicochemical properties. For all bacterial strains, the highest retention (up to 100% or log 6 reduction) at >50 L/h∗bar∗m$^2$ flow rate was achieved with a 4-layer gradient-structured membrane containing different aCNF content, thereby matching the performance of industrial polymeric filters used for removing bacteria.

• Jezik: Angleški jezik
• Ključne besede: fibrous membrane, cationised cellulose nanofibrils, amino-hydrophobised cellulose nanofibrils, antibacterial activity, multi-layer structure, flux, bacteria retention
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 23 str.
• Številčenje: Vol. 13, iss. 3, art. 284
• PID: 20.500.12556/RUL-155611
• UDK: 577:620.3
• ISSN pri članku: 2077-0375
• DOI: 10.3390/membranes13030284
• COBISS.SI-ID: 144515331
• Datum objave v RUL: 08.04.2024

Gradivo je del revije

• Naslov: Membranes
• Skrajšan naslov: Membranes
• Založnik: MDPI
• ISSN: 2077-0375
• COBISS.SI-ID: 519125529

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: fibrozne membrane, kationizirani celulozni nanofibrili, amino-hidrofobizirani celulozni nanofibrili, protibakterijska aktivnost, večplastna struktura, tok, zadrževanje bakterij