Amyloid fibrils of stefin B show anisotropic properties

Žganec, Matjaž (avtor), Taler-Verčič, Ajda (avtor), Muševič, Igor (avtor), Škarabot, Miha (avtor), Žerovnik, Eva (avtor)

Human stefin B, a member of the cystatin family of cysteine protease inhibitors, tends to form amyloid fibrils under relatively mild conditions, which is why it is used as a model protein to study amyloid fibrillation. Here, we show for the first time that bundles of amyloid fibrils, i.e., helically twisted ribbons, formed by human stefin B exhibit birefringence. This physical property is commonly observed in amyloid fibrils when stained with Congo red. However, we show that the fibrils arrange in regular anisotropic arrays and no staining is required. They share this property with anisotropic protein crystals, structured protein arrays such as tubulin and myosin, and other anisotropic elongated materials, such as textile fibres and liquid crystals. In certain macroscopic arrangements of amyloid fibrils, not only birefringence is observed, but also enhanced emission of intrinsic fluorescence, implying a possibility to detect amyloid fibrils with no labels by using optical microscopy. In our case, no enhancement of intrinsic tyrosine fluorescence was observed at 303 nm; instead, an additional fluorescence emission peak appeared at 425 to 430 nm. We believe that both phenomena, birefringence and fluorescence emission in the deep blue, should be further explored with this and other amyloidogenic proteins. This may allow the development of label-free detection methods for amyloid fibrils of different origins.

• Jezik: Angleški jezik
• Ključne besede: nano-sized object, bundle of amyloid fibrils, birefringence, intrinsic fluorescence, label-free imaging
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FMF - Fakulteta za matematiko in fiziko ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 10 str.
• Številčenje: Vol. 24, iss. 4, art. 3737
• PID: 20.500.12556/RUL-155598
• UDK: 577.112
• ISSN pri članku: 1661-6596
• DOI: 10.3390/ijms24043737
• COBISS.SI-ID: 142086659
• Datum objave v RUL: 08.04.2024

ŽGANEC, Matjaž, TALER-VERČIČ, Ajda, MUŠEVIČ, Igor, ŠKARABOT, Miha in ŽEROVNIK, Eva, 2023, Amyloid fibrils of stefin B show anisotropic properties. International journal of molecular sciences [na spletu]. 2023. Vol. 24, no. 4,  3737. [Dostopano 4 april 2025]. DOI 10.3390/ijms24043737. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=155598

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: delci nanovelikosti, skupki amiloidnih fibril, dvolomnost, intrinzična fluorescenca, slikanje brez označevanja

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J7-4050
• Naslov: Oligomeri amiloidogenih proteinov od a do ž; biofizikalne lastnosti, struktura, funkcija in medsebojne interakcije

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0140
• Naslov: Proteoliza in njena regulacija pri zdravju in boleznih