A synergistic anti-bacterial and anti-adhesion activity of tea tree (Melaleuca alternifolia) and lemon eucalyptus tree (Eucalyptus citriodora Hook) essential oils on Legionella pneumophila

Oder, Martina (avtor), Piletić, Kaća (avtor), Fink, Rok (avtor), Marijanović, Zvonimir (avtor), Krištof, Romana (avtor), Bićanić, Lucija (avtor), Tomić Linšak, Dijana (avtor), Gobin, Ivana (avtor)

Legionella pneumophila is a Gram-negative bacterial pathogen that colonizes natural and artificial water systems and has the ability to form a biofilm. The biofilm protects L. pneumophila from various environmental factors and makes it more resistant to chlorine-based disinfectants. This study investigated the anti-bacterial properties of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel) oil and lemon eucalyptus tree (Eucalyptus citriodora Hook) essential oils (EOs) and their synergistic, additive inhibitory and anti-adhesive effects against L. pneumophila biofilm formation on polystyrene. The minimum effective concentration (MEC) for tea tree is 12.8 mg ml$^{−1}$ and for lemon eucalyptus tree EO 6.4 mg ml$^{−1}$. In the checkerboard assay, different combinations of these two EO show synergistic and additive anti-microbial activity. The minimum anti-adhesive concentration (MAC) for tea tree is 12.8 mg ml$^{−1}$ and for lemon eucalyptus tree EO 6.4 mg ml$^{−1}$. A combination of 3.2 mg ml$^{−1}$ tea tree EO and 0.8 mg ml$^{−1}$ lemon eucalyptus tree EO showed the strongest anti-adhesive effect against L. pneumophila on polystyrene. The tested oils and their combination showed intriguing potential to inhibit L. pneumophila biofilm formation.

• Jezik: Angleški jezik
• Ključne besede: anti-Legionella activity, anti-adhesion, mass spectrometry, essential oils, checkerboard assay
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 54-63
• Številčenje: Vol. 40, no. 1
• PID: 20.500.12556/RUL-154949
• UDK: 579.2:543.51:614
• ISSN pri članku: 0892-7014
• DOI: 10.1080/08927014.2024.2310482
• COBISS.SI-ID: 188405251
• Datum objave v RUL: 11.03.2024

Gradivo je del revije

• Naslov: Biofouling
• Skrajšan naslov: Biofouling
• Založnik: Taylor & Francis
• ISSN: 0892-7014
• COBISS.SI-ID: 15611141

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: BI-HR/20-21-005

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: University of Rijeka
• Številka projekta: UNIRI-BIOMED-18-171