A simplified nasopharyngeal swab collection procedure for minimizing patient discomfort while retaining sample quality

Uršič, Tina (avtor), Kogoj, Rok (avtor), Šikonja, Jaka (avtor), Jevšnik Virant, Monika (avtor), Petrovec, Miroslav (avtor)

A nasopharyngeal swab (NPS) is the most frequently collected sample type when molecular diagnosis of respiratory viruses, including SARS CoV-2, is required. An optimal collection technique would provide sufficient sample quality for the diagnostic process and would minimize the discomfort felt by the patient. This study compares a simplified NPS collection procedure with only one rotation of the swab to a more standard procedure with five rotations. Swabs were collected from 76 healthy volunteers by the same healthcare professional on 2 consecutive days at a similar hour to minimize variability. The number of Ubiquitin C copy number per sample was measured by real-time quantitative PCR and patient discomfort was assessed by questionnaire. No statistically significant difference (p = 0.15) was observed in the Ubiquitin C copy number per sample between a NPS collected with one rotation (5.2 ± 0.6 log UBC number copies/sample) or five rotations (5.3 ± 0.5 log UBC number copies/sample). However, a statistically significant difference was observed in discomfort between these two procedures, the second being much more uncomfortable. Additional analysis of the results showed a weak correlation between discomfort and the number of human cells recovered (Spearman's rho = 0.202) and greater discomfort in younger people. The results of this study show that a NPS collected with one slow rotation has the same quality as a NPS collected with five rotations. However, the collection time is shorter and, most importantly, less unpleasant for patients.

• Jezik: Angleški jezik
• Ključne besede: nasopharyngeal swab, collection procedure, sample quality, discomfort score, Ubiquitin C
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 4 str.
• Številčenje: Vol. 11, art. 1066934
• PID: 20.500.12556/RUL-154018
• UDK: 616-07
• ISSN pri članku: 2296-2565
• DOI: 10.3389/fpubh.2023.1066934
• COBISS.SI-ID: 146264835
• Datum objave v RUL: 19.01.2024

Gradivo je del revije

• Naslov: Frontiers in public health
• Skrajšan naslov: Front. public health
• Založnik: Frontiers Media
• ISSN: 2296-2565
• COBISS.SI-ID: 523096857

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: nazofaringealni bris, postopek odvzema, kakovost vzorca

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0083
• Naslov: Odnosi parazitskega obstajanja