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Model-based design of continuous biotransformation in a microscale bioreactor with yeast cells immobilized in a hydrogel film : research data underlying the article
ID Menegatti, Tadej (Avtor), ID Plazl, Igor (Avtor), ID Žnidaršič Plazl, Polona (Avtor)

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Izvleček
Miniaturized flow reactors with immobilized biocatalysts offer enormous potential for process intensification. They enable long-term use of biocatalysts, continuous operation that significantly exceeds the performance of batch processes, and efficient mass and heat transfer leading to highly controlled reaction conditions. Despite their increasing use in biocatalytic processes, optimization of reactor design and operating conditions based on a mathematical description is very rare. This work aims to fill this gap by developing and validating a mathematical model for the continuous biotransformation process in a microreactor between two plates with immobilized whole cells in hydrogel layers on the bottom and top of the reactor. A biocatalytic production of L-malic acid by fumaric acid hydration using permeabilized Saccharomyces cerevisiae whole cells was used as a model reaction. The diffusivity of substrate and product in a liquid phase and in a copolymer hydrogel layer, as well as the reaction kinetic parameters considering the Michaelis-Menten kinetics of the reversible enzymatic reaction, were estimated in initial batch experiments. The results obtained in a continuously operated microbioreactor with immobilized whole cells at different fumaric acid concentrations and flow rates were in excellent agreement with the predictions of the developed mathematical model encompassing transport phenomena and reaction kinetics. Based on the validated model and using time scale analysis with characteristic times, the optimal process and operating conditions for the developed microbioreactor system were determined. By reducing the channel thickness to 200 um and increasing the gel thickness to 900 microns, we were able to increase the complete conversion of the substrate with a residence time of less than 40 minutes.

Jezik:Angleški jezik
Ključne besede:microreactor, mathematical model, immobilization, whole-cell biocatalysis, continuous biotransformation, time-scale analysis
Organizacija:FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Leto izida:2023
PID:20.500.12556/RUL-151814 Povezava se odpre v novem oknu
Metode zbir. podat.:Meritve in testi
Datum objave v RUL:20.10.2023
Število ogledov:809
Število prenosov:166
Metapodatki:XML DC-XML DC-RDF
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Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:mikroreaktor, matematični model, imobilizacija, biokataliza s celimi celicami, kontinuirna biotransformacija, time-scale analiza

Projekti

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P2-0191
Naslov:Kemijsko inženirstvo

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:J4-4562
Naslov:Intenzifikacija biokatalitskih procesov z uporabo evtektičnih topil v mikropretočnih sistemih za trajnostno valorizacijo odpadkov - BioInDES

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Program financ.:Young Researchers

Financer:EC - European Commission
Program financ.:H2020
Številka projekta:811040
Naslov:Chair Of Micro Process Engineering and TEchnology
Akronim:COMPETE

Financer:Drugi - Drug financer ali več financerjev
Program financ.:M-ERA.NET
Številka projekta:337610
Naslov:Neoteric Biomaterials for hIPSCs Monitorized Differentiation to RGCs: Creation, Microfabrication & Microfluidics
Akronim:Biomat4eye

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