Primernost indikatorske metode za določanje navidezne izkoristljivosti hranil pri pitovnih piščancih

Kavčič, Aljaž (avtor), Levart, Alenka (mentor), Leskovec, Jakob (komentor)

Na pitovnih piščancih smo ugotavljali vpliv vročinskega stresa na izkoristljivost hranil, merjeno z indikatorsko metodo z dvema različnima indikatorjema. Piščance smo razdelili v dva prostora v hlevu s po osem oddelki (10 piščancev na oddelek; n=160). Polovici oddelkov smo 21. dan poskusa začeli za 10 ur dnevno zviševati temperaturo v prostoru na 30-32 °C (skupina vroče okolje; VO), druga polovica piščancev pa je bila nameščena v termonevtralnem okolju (TNO). Tri dni pred zaključkom poskusa smo živalim v krmo dodali titanov oksid (TiO2) kot eksterni marker. Na 42. dan smo zbrali izločke ter izračunali navidezno izkoristljivost hranljivih snovi s pomočjo indikatorske metode s TiO2 (NI-Ti). Kot indikator smo uporabili še interni marker lignin, določen kot v kislini netopen lignin (KDL), ter izračunali navidezno izkoristljivost (NI-KDL). Podatke smo obdelali s statističnim programom SAS s proceduro GLM. Pri analizi krme smo ugotovili zelo velike vsebnosti rudninskih snovi, še posebej mikrorudnin, kar je bila posledica kontaminacije surovin že pred pripravo krme. Statističnih razlik med skupinami TNO in VO nismo zasledili, ne glede na uporabljeno indikatorsko metodo. Je pa prišlo do razlike pri primerjavi indikatorskih metod, in sicer so bile praktično vse vrednosti NI-KDL manjše kot vrednosti po NI-Ti metodi. Sklepamo lahko, da je prišlo do kontaminacije izločkov z nastiljem, kar je povečalo vsebnost KDL v izločkih in s tem zmanjšalo izračunano izkoristljivost pri metodi NI-KDL.

• Jezik: Slovenski jezik
• Ključne besede: prehrana, pitovni piščanci, prebavljivost, navidezna izkoristljivost, indikatorska metoda, titanov oksid
• Vrsta gradiva: Diplomsko delo/naloga
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2023
• PID: 20.500.12556/RUL-148374
• COBISS.SI-ID: 161815555
• Datum objave v RUL: 20.08.2023

KAVČIČ, Aljaž, 2023, Primernost indikatorske metode za določanje navidezne izkoristljivosti hranil pri pitovnih piščancih [na spletu]. Diplomsko delo. [Dostopano 21 april 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=148374

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Assessment of the suitability of an indicator method for the determination of apparent nutrient utilization in broiler chickens

Izvleček

The effects of heat stress on nutrient utilization were studied in broiler chickens using indicator method with two different markers. Chickens were divided into two research facility (RF) rooms with eight compartments (10 chickens per compartment; n=160). Beginning on day 21 of the experiment, chickens in half of the compartments (RF room 1) were exposed to a temperature of 30-32°C for 10 hours per day (warm environment group; VO), while the other half of the chickens (RF room 2) were housed in a thermoneutral environment (TNO). Three days before the end of the experiment, titanium oxide (TiO2) was added to the feed as an external marker. On day 42, the excreta were collected and the apparent utilization of nutrients was calculated using the TiO2 indicator method (NI-Ti). The internal marker lignin, defined as acid detergent lignin (ADL), was also used as an indicator and apparent nutrient utilization (NI-KDL) was calculated. Data were processed using SAS statistical software with the GLM procedure. In the analysis of the feed, we found very high mineral contents, especially microminerals, which was due to contamination of the raw materials before feed preparation. No statistical differences were found in apparent nutrient utilization between the TNO and VO group, regardless of the indicator method used. However, there were differences in nutrient utilization when comparing indicator methods, and virtually all values of NI-KDL were lower than those of the NI-Ti method. This suggests that the excreta were contaminated with litter, which increased the ADL content of the excreta and thus decreased the calculated apparent nutrient utilization of the NI-ADL method.

• Ključne besede: nutrition, broiler chickens, digestibility, apparent nutrient utilisation, indicator method, titan oxide