ATR-FTIR spectroscopy as a pre-screening technique for the PMI assessment and DNA preservation in human skeletal remains : a review

Leskovar, Tamara (avtor), Zupanič-Pajnič, Irena (avtor), Jerman, Ivan (avtor), Črešnar, Matija (avtor)

Skeletal remains from archaeological and forensic contexts are commonly used for various analyses, including DNA. Our research investigates relatively fast and inexpensive ATR-FTIR spectroscopy with further data manipulation as a potential pre-screening technique for assessing the preservation state of skeletal remains. We seek to correlate spectra and chemometric indices with the biological, chemical, and physical composition of the skeletal tissue, and the quality and quantity of DNA preserved in the tissue, also considering the skeletal element, absolute age, and environmental conditions to which the remains have been exposed. Our research focuses on human bones and teeth derived from archaeological, WWII and forensic contexts. The samples are cleaned and pulverized according to established methodological procedures for DNA extraction. DNA is extracted and quantified, while the remaining powder is analysed by ATR-FTIR spectroscopy. The obtained spectra are manipulated to extract the chemometric indices, which are the base for statistical analyses, machine learning, and prediction algorithms. A compilation of results from several studies shows that ATR-FTIR spectra present a solid base for researchers to better understand the preservation state of phosphates, carbonates, and collagen in the skeletal tissues, assessment of post-mortem interval, and the preservation of the DNA. Although many questions still need to be answered, ATR-FTIR spectroscopy with further data manipulation has a high potential for research of taphonomic changes, PMI and DNA preservation in human skeletal remains.

• Jezik: Angleški jezik
• Ključne besede: human remains, ATR-FTIR spectroscopy, preservation state, pre-screening method
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: FF - Filozofska fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: Str. 56-64
• Številčenje: Vol. 660
• PID: 20.500.12556/RUL-145905
• UDK: 615.4
• ISSN pri članku: 1040-6182
• DOI: 10.1016/j.quaint.2022.09.004
• COBISS.SI-ID: 123458819
• Datum objave v RUL: 17.05.2023

Gradivo je del revije

• Naslov: Quaternary international
• Skrajšan naslov: Quat. int.
• Založnik: Elsevier
• ISSN: 1040-6182
• COBISS.SI-ID: 15081989

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: človeški ostanki, ATR-FTIR spektroskopija, stanje ohranjenosti