Vpliv COVID-19 na koncentracije citokinov, rastnih dejavnikov in kemokinov v plazmi krvodajalcev

Kolenc, Ana (avtor), Maličev, Elvira (mentor)

COVID-19 je bolezen, ki jo povzroča SARS-CoV-2. Okužbo spremljajo povišana telesna temperatura, kašelj in utrujenost, pa tudi izkašljevanje sputuma, glavobol in drugi bolj specifični simptomi. Bolniki s težjo obliko COVID-19 imajo simptome hudega sistemskega vnetja, ob čemer se sproščajo velike količine citokinov, kemokinov in rastnih dejavnikov. Njihova plazma pa poleg mnogih bioaktivnih molekul vsebuje tudi protitelesa proti SARS-CoV-2, zato je bila infuzija hiperimunske prebolevniške plazme ena izmed terapij, predlaganih za zdravljenje okužbe z SARS-CoV-2. Klinične raziskave prebolevniške plazme so pokazale, da so ključni čas aplikacije plazme, stanje bolnika in titer protiteles v plazmi, manj znanega pa je o vplivu ostalih sestavin krvne plazme na bolnika. V magistrski nalogi smo primerjali koncentracijo vnetnih in protivnetnih bioaktivnih molekul pri krvodajalcih, ki so preboleli COVID-19, in koncentracijo vnetnih in protivnetnih bioaktivnih molekul pri krvodajalcih, ki bolezni niso preboleli. Preverili smo tudi odvisnost ravni analitov glede na čas, ki je pretekel od prvih znakov bolezni do darovanja krvi, korelacijo med posameznimi citokini in povezanost ravni citokinov s titri protiteles IgG proti SARS-CoV-2 in nevtralizirajočimi protitelesi. V nalogi nismo odkrili statistično značilnih razlik ali izrazitih korelacij, ki bi omejevale uporabo hiperimunske prebolevniške plazme za terapijo bolnikov s COVID-19.

• Jezik: Slovenski jezik
• Ključne besede: citokini, COVID-19, hiperimunska prebolevniška plazma, protitelesa, SARS-CoV-2
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2023
• PID: 20.500.12556/RUL-145380
• COBISS.SI-ID: 149856771
• Datum objave v RUL: 20.04.2023

KOLENC, Ana, 2023, Vpliv COVID-19 na koncentracije citokinov, rastnih dejavnikov in kemokinov v plazmi krvodajalcev [na spletu]. Magistrsko delo. [Dostopano 5 maj 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=145380

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: The effect of COVID-19 on the concentrations of cytokines, growth factors and chemokines in the plasma of blood donors

Izvleček

COVID-19 is a disease caused by SARS-CoV-2. The infection is associated with an increased body temperature, cough and fatigue, expectoration and headache. Patients with an extreme form of COVID-19, exhibit symptoms of severe systemic inflammation, releasing large amounts of cytokines, chemokines and growth factors. In addition, their plasma also contains antibodies against SARS-CoV-2, which is the reason for the infusion of hyperimmune convalescent plasma being one of the therapies proposed for the treatment of the infection. Clinical studies have shown that the timing of plasma application, the patient's condition and the plasma antibody titer, is crucial. However, less is known about the effect of other blood plasma components. We compared the concentration of inflammatory and anti-inflammatory bioactive molecules in blood donors that recovered from COVID-19 and their concentration in blood donors that did not yet have the disease. We also examined the dependence of analyte levels on the elapsed time between the initial signs of disease and blood donation, the correlation between individual cytokines and the association of cytokine levels with IgG antibody titers against SARS-CoV-2 and neutralizing antibodies. We concluded that there were no statistically significant differences or correlations, that would limit the use of hyperimmune convalescent plasma for the treatment of COVID-19 patients.

• Ključne besede: antibodies, convalescent plasma, COVID-19, cytokines, SARS-CoV-2