Ceramide phosphoethanolamine as a possible marker of periodontal disease

Grundner, Maja (avtor), Munjaković, Haris (avtor), Tori, Tilen (avtor), Sepčić, Kristina (avtor), Gašperšič, Rok (avtor), Oblak, Čedomir (avtor), Seme, Katja (avtor), Guella, Graziano (avtor), Trenti, Francesco (avtor), Skočaj, Matej (avtor)

Periodontal disease is a chronic oral inflammatory disorder initiated by pathobiontic bacteria found in dental plaques—complex biofilms on the tooth surface. The disease begins as an acute local inflammation of the gingival tissue (gingivitis) and can progress to periodontitis, which eventually leads to the formation of periodontal pockets and ultimately results in tooth loss. The main problem in periodontology is that the diagnosis is based on the assessment of the already obvious tissue damage. Therefore, it is necessary to improve the current diagnostics used to assess periodontal disease. Using lipidomic analyses, we show that both crucial periodontal pathogens, Porphyromonas gingivalis and Tannerella forsythia, synthesize ceramide phosphoethanolamine (CPE) species, membrane sphingolipids not typically found in vertebrates. Previously, it was shown that this particular lipid can be specifically detected by an aegerolysin protein, erylysin A (EryA). Here, we show that EryA can specifically bind to CPE species from the total lipid extract from P. gingivalis. Furthermore, using a fluorescently labelled EryA-mCherry, we were able to detect CPE species in clinical samples of dental plaque from periodontal patients. These results demonstrate the potential of specific periodontal pathogen-derived lipids as biomarkers for periodontal disease and other chronic inflammatory diseases.

• Jezik: Angleški jezik
• Ključne besede: ceramide phosphoethanolamine, periodontal disease, aegerolysin, biomarker, erylysin A, Porphyromonas gingivalis
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 19 str.
• Številčenje: Vol. 12, iss. 7, art. 655
• PID: 20.500.12556/RUL-145170
• UDK: 616.31
• ISSN pri članku: 2077-0375
• DOI: 10.3390/membranes12070655
• COBISS.SI-ID: 113667587
• Datum objave v RUL: 12.04.2023

Gradivo je del revije

• Naslov: Membranes
• Skrajšan naslov: Membranes
• Založnik: MDPI
• ISSN: 2077-0375
• COBISS.SI-ID: 519125529

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ceramid fosfoetanolamin, parodontalna bolezen, egerolizin

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0207
• Naslov: Toksini in biomembrane

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-1747
• Naslov: Egerolizinski proteini kot nova teranostična sredstva pri parodontalni bolezni in protetični oskrbi