Identifikacija in karakterizacija bakterij ter proučevanje njihovih sinergističnih učinkov pri pospeševanju rasti kumare (Cucumis sativus)

Miška, Matija (avtor), Štefanič, Polonca (mentor)

Potreba po učinkovitih biognojilih se veča zaradi širjenja okolju prijaznih delovnih praks v kmetijstvu. Za boljšo uporabo biognojil potrebujemo raziskave na področju mehanizmov delovanja pospeševalcev rasti rastlin ter njihovih medsebojnih interakcijah v pripravkih. V tem delu smo sestavili postopek za presejanje potencialnih bakterijskih pospeševalcev rasti rastlin (PGPB), ki spadajo v rod rodu Bacillus. Postopek je sestavljen iz identifikacije in karakterizacije lastnosti, ki so povezane s pospeševanjem rasti rastlin (PGP). Postopek smo uspešno uporabili za oženje zbirke 269-ih izolatov na 46 potencialnih PGPB. Te smo ločili na »kin« in »non-kin« seve, glede na njihov fenotip sorodstvenega razlikovanja (KD) s komercialnim sevom B. velezenzis SQR9. Sestavili smo eksperimentalni »kin« in »non-kin« mešanici biognojila, kar nam je omogočilo vpogled v vpliv KD na PGP sposobnost mešanice sevov, ter ta biognojila skupaj z negativno kontrolo (NK) in pozitivno kontrolo SQR9 (PK) preizkušali na rastlinah kumarah. Rastline, inokulirane z eksperimentalnimi biognojili so hitreje ter obilneje cvtele, v primerjavi z NK in PK. Pričakovali smo boljše delovanje »kin« biognojila, zaradi odsotnosti antagonizma med sevi v mešanici. Čeprav nismo opazili izrazito povečane rasti rastlin, smo odkrili povezave med biognojilom in koreninskim sistemom. Mešanica »kin« sevov je na kumarah v primerjavi z ostalimi inokulumi izrazito spodbudila stransko rast korenin in s tem povečanje koreninskega sistema.

• Jezik: Slovenski jezik
• Ključne besede: pospeševanje rasti rastlin, bakterije, Bacillus, biognojila, sorodstveno razlikovanje
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2023
• PID: 20.500.12556/RUL-144597
• COBISS.SI-ID: 143904515
• Datum objave v RUL: 03.03.2023

MIŠKA, Matija, 2023, Identifikacija in karakterizacija bakterij ter proučevanje njihovih sinergističnih učinkov pri pospeševanju rasti kumare (Cucumis sativus) [na spletu]. Magistrsko delo. [Dostopano 3 maj 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=144597

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Identification, characterisation and the influence of PGPR synergism on cucumber growth (Cucumis sativus)

Izvleček

The need for effective biofertilizers is increasing due to the spread of environmentally conscious agricultural work practices. For better usage of biofertilizers, we need further research into the mechanisms of plant growth promoters and their social interactions in commercial products. In this work, we have compiled a procedure for the screening of potential bacterial plant growth promoters (PGPB), belonging to the genus Bacillus. The process consists of the identification of isolates and the characterization of traits, associated with plant growth promotion (PGP). The procedure was successfully used to narrow down a collection of 269 isolates to 46 potential PGPBs. These were separated into “kin” and “non-kin” strains, based on their kin differentiation (KD) phenotype with the commercial PGPB strain B. velezensis SQR9. We composed experimental “kin" and "non-kin" mixtures of biofertilizers, which gave us an insight into the influence of KD on the PGP ability of the biofertilizers, and tested those biofertilizers on cucumber plants, compared to the negative control (NK) and the positive control SQR9 (PK). Plants, inoculated with experimental biofertilizers, flowered faster and more abundantly compared to NK and PK. We expected a better performance of "kin" biofertilizer, due to the absence of antagonism between the strains in the mixture. Although we did not observe a significant increase in plant growth, we discovered a connection between the biofertilizer and the root system. The mixture of "kin" strains significantly stimulated the lateral growth of roots and thus the increase in overall size of the root system on cucumbers, compared to the other inoculums.

• Ključne besede: plant growth promotion, bacteria, Bacillus, biofertilizer, kin discrimination