Covalently conjugated NOD2/TLR7 agonists are potent and versatile immune potentiators

Guzelj, Samo (avtor), Weiss, Matjaž (avtor), Slütter, Bram (avtor), Frkanec, Ruža (avtor), Jakopin, Žiga (avtor)

The success of vaccination with subunit vaccines often relies on the careful choice of adjuvants. There is great interest in developing new adjuvants that can elicit a cellular immune response. Here, we address this challenge by taking advantage of the synergistic cross-talk between two pattern recognition receptors: nucleotide-binding oligomerization-domain-containing protein 2 (NOD2) and Toll-like receptor 7 (TLR7). We designed two conjugated NOD2/TLR7 agonists, which showed potent immunostimulatory activities in human primary peripheral blood mononuclear cells and murine bone-marrow-derived dendritic cells. One of these, 4, also generated a strong antigen-specific immune response in vivo, with a Th1-polarized profile. Importantly, our study shows that novel NOD2/TLR7 agonists elicit sophisticated and fine-tuned immune responses that are inaccessible to individual NOD2 and TLR7 agonists.

• Jezik: Angleški jezik
• Ključne besede: immunostimulatory activity, vaccines, immune responses, pattern recognition receptors, synthesis of NOD2 agonist, Toll-like receptor 7
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: Str. 15085–15101
• Številčenje: Vol. 65, iss. 22
• PID: 20.500.12556/RUL-143233
• UDK: 615.371:616-097
• ISSN pri članku: 1520-4804
• DOI: 10.1021/acs.jmedchem.2c00808
• COBISS.SI-ID: 128189187
• Datum objave v RUL: 09.12.2022

Gradivo je del revije

• Naslov: Journal of medicinal chemistry
• Skrajšan naslov: J. med. chem.
• Založnik: American Chemical Society
• ISSN: 1520-4804
• COBISS.SI-ID: 512806681

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: imunostimulatorna aktivnost, imunski odziv, receptorji za prepoznavanje vzorcev, sinteza agonista NOD2, receptor TLR7, cepiva