The influence of excipients on the viscosity of monoclonal antibody solutions

Hribar-Lee, Barbara (avtor)

The aggregation propensity of monoclonal antibodies can be modified by adding different cosolutes into the solution. A simple coarse-grained model in the combination with the thermodynamic perturbation theory was used to predict cluster distribution and viscosity of the solutions of IgG4 monoclonal anibody in the presence of L-Arginine Hydrochloride. The data were analysed using binding polynomial to describe the binding of cosolute (Arginine) to the antibody molecule. The results show that by binding to the antibody molecule the cosolute occupies some of the binding sites of the antibody, and in this way reduces the amount of binding sites available to other antibody molecules. The aggregation propensity of the antibody molecules is therefore reduced.

• Jezik: Angleški jezik
• Ključne besede: monoclonal antibody, aggregation, viscosity, cosolutes, thermodynamic perturbation theory, binding polynomial
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 5 str.
• Številčenje: Vol. 366, art. 120349
• PID: 20.500.12556/RUL-142787
• UDK: 544.35:577.322
• ISSN pri članku: 0167-7322
• DOI: 10.1016/j.molliq.2022.120349
• COBISS.SI-ID: 121626627
• Datum objave v RUL: 25.11.2022

Gradivo je del revije

• Naslov: Journal of molecular liquids
• Skrajšan naslov: J. mol. liq.
• Založnik: Elsevier
• ISSN: 0167-7322
• COBISS.SI-ID: 15382277

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: monoklonska protitelesa, agregacija, viskoznost, sotopljenci, termodinamska perturbacijska teorija, statistična vsota vezanja

Projekti

• Financer: NIH - National Institutes of Health
• Številka projekta: RM1GM135136