Effect of UV light and sodium hypochlorite on formation and destruction of Pseudomonas fluorescens biofilm in vitro

Sigler Zekanović, Melani (avtor), Begić, Gabrijela (avtor), Mežnarić, Silvestar (avtor), Jelovica Badovinac, Ivana (avtor), Krištof, Romana (avtor), Tomić Linšak, Dijana (avtor), Gobin, Ivana (avtor)

Pseudomonas fluorescens is one of the first colonizers of bacterial biofilm in water systems and a member of opportunistic premise plumbing pathogens (OPPPs). The aim of this study was to examine the effect of UV light and sodium hypochlorite on the formation and destruction of mature P. fluorescens biofilm on ceramic tiles. Planktonic bacteria or bacteria in mature biofilm were exposed to UV light (254 nm) for 5, 20 s. and to 0.4 mg/L sodium hypochlorite for 1 min. Mature biofilm was also exposed to increased concentration of sodium hypochlorite of 2 mg/L for 0.5, 1 and 2 h and combined with UV. Prolonged action of sodium hypochlorite and an increase in its concentration in combination with UV gave the best results in the inhibition of biofilm formation after the pretreatment and destruction of mature biofilm. The effect of hyperchlorination in combination with UV radiation shows better results after a long exposure time, although even after 120 min there was no completely destroyed biofilm. Furthermore, the mechanism of the effect of combined methods should be explored as well as the importance of mechanical cleaning that is crucial in combating bacterial biofilm in swimming pools.

• Jezik: Angleški jezik
• Ključne besede: biofilm, disinfection, opportunistic pathogens, Pseudomonas fluorescens, UV light
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 01.01.2022
• Leto izida: 2022
• Št. strani: 15 str.
• Številčenje: Vol. 10, no. 10, art. 1901
• PID: 20.500.12556/RUL-142784
• UDK: 614:628.166
• ISSN pri članku: 2227-9717
• COBISS.SI-ID: 130886147
• Datum objave v RUL: 25.11.2022

Gradivo je del revije

• Naslov: Processes
• Skrajšan naslov: Processes
• Založnik: MDPI
• ISSN: 2227-9717
• COBISS.SI-ID: 523353113

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Ni določen
• Ključne besede: biofilm, dezinfekcija, oportunistični patogeni, Pseudomonas fluorescens, UV svetloba