First detection of honeybee pathogenic viruses in butterflies

Pislak, Metka (avtor), Glavan, Gordana (avtor), Verovnik, Rudi (avtor), Šimenc Kramar, Laura (avtor), Toplak, Ivan (avtor)

Several pathogens are important causes of the observed pollinator decline, some of which could be transmitted between different pollinator species. To determine whether honeybee viruses can be transmitted to butterflies, a total of 120 butterflies were sampled at four locations in Slovenia. At each location, butterflies from three families (Pieridae, Nymphalidae, Hesperiidae/Lycenidae) and Carniolan honeybees (Apis mellifera carnica) were collected. The RNA of six honeybee viruses, i.e., acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus A (DWV-A), Sacbrood bee virus (SBV), and Lake Sinai virus 3 (LSV3), was detected by a specific quantitative method (RT-PCR). The presence of ABPV, BQCV, LSV3, and SBV was detected in both butterflies and honeybees. All butterfly and bee samples were negative for CBPV, while DWV-A was detected only in honeybees. The viral load in the positive butterfly samples was much lower than in the positive bee samples, which could indicate that butterflies are passive carriers of bee viruses. The percentage of positive butterfly samples was higher when the butterflies were collected at sampling sites with a higher density of apiaries. Therefore, we believe that infected bees are a necessary condition for the presence of viruses in cohabiting butterflies. This is the first study on the presence of pathogenic bee viruses in butterflies.

• Jezik: Angleški jezik
• Ključne besede: butterflies, honeybees, viruses, pathogens transmission, bees, virology
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 12 str.
• Številčenje: Vol. 13, iss. 10, art. 925
• PID: 20.500.12556/RUL-142110
• UDK: 578
• ISSN pri članku: 2075-4450
• DOI: 10.3390/insects13100925
• COBISS.SI-ID: 126058243
• Datum objave v RUL: 20.10.2022

PISLAK, Metka, GLAVAN, Gordana, VEROVNIK, Rudi, ŠIMENC KRAMAR, Laura in TOPLAK, Ivan, 2022, First detection of honeybee pathogenic viruses in butterflies. Insects [na spletu]. 2022. Vol. 13, no. 10,  925. [Dostopano 5 maj 2025]. DOI 10.3390/insects13100925. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=142110

Gradivo je del revije

• Naslov: Insects
• Založnik: MDPI
• ISSN: 2075-4450
• COBISS.SI-ID: 519122457

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0184
• Naslov: Integrativna zoologija in speleobiologija