The Bacillus phage SPβ and its relatives : a temperate phage model system reveals new strains, species, prophage integration loci, conserved proteins and lysogeny management components

Kohm, Katharina (avtor), Floccari, Valentina Andrea (avtor), Lutz, Veronika T. (avtor), Nordmann, Birthe (avtor), Mittelstädt, Carolin (avtor), Poehlein, Anja (avtor), Dragoš, Anna (avtor), Commichau, Fabian M. (avtor), Hertel, Robert (avtor)

The Bacillus phage SPβ has been known for about 50 years, but only a few strains are available. We isolated four new wild-type strains of the SPbeta species. Phage vB_BsuS-Goe14 introduces its prophage into the spoVK locus, previously not observed to be used by SPβ-like phages. Sequence data revealed the genome replication strategy and the genome packaging mode of SPβ-like phages. We extracted 55 SPβ-like prophages from public Bacillus genomes, thereby discovering three more integration loci and one additional type of integrase. The identified prophages resemble four new species clusters and three species orphans in the genus Spbetavirus. The determined core proteome of all SPβ-like prophages consists of 38 proteins. The integration cassette proved to be not conserved, even though, present in all strains. It consists of distinct integrases. Analysis of SPβ transcriptomes revealed three conserved genes, yopQ, yopR, and yokI, to be transcribed from a dormant prophage. While yopQ and yokI could be deleted from the prophage without activating the prophage, damaging of yopR led to a clear-plaque phenotype. Under the applied laboratory conditions, the yokI mutant showed an elevated virion release implying the YokI protein being a component of the arbitrium system.

• Jezik: Angleški jezik
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Datum objave: 15.03.2022
• Leto izida: 2022
• Št. strani: Str. 2098-2118
• Številčenje: Vol. 24, no. 4
• PID: 20.500.12556/RUL-138602
• UDK: 579.852.11:578.347:575.111
• ISSN pri članku: 1462-2912
• DOI: 10.1111/1462-2920.15964
• COBISS.SI-ID: 101899011
• Datum objave v RUL: 02.08.2022

KOHM, Katharina, FLOCCARI, Valentina Andrea, LUTZ, Veronika T., NORDMANN, Birthe, MITTELSTÄDT, Carolin, POEHLEIN, Anja, DRAGOŠ, Anna, COMMICHAU, Fabian M. in HERTEL, Robert, 2022, The Bacillus phage SPβ and its relatives : a temperate phage model system reveals new strains, species, prophage integration loci, conserved proteins and lysogeny management components. Environmental microbiology [na spletu]. 2022. Vol. 24, no. 4, p. 2098–2118. [Dostopano 10 april 2025]. DOI 10.1111/1462-2920.15964. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=138602

Gradivo je del revije

• Naslov: Environmental microbiology
• Skrajšan naslov: Environ. microbiol.
• Založnik: Society for Applied Microbiology and John Wiley & Sons
• ISSN: 1462-2912
• COBISS.SI-ID: 626197

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 02.08.2022

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Bacillus subtilis, profagi, divji tip sevov, lizogenija, genomika

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: N1-0177
• Naslov: Nadzor bakterijske fiziologije in interakcij s profagi