Structural polymorphism of coiled-coils from the stalk domain of SARS-CoV-2 spike protein

Živič, Zala (avtor), Strmšek, Žiga (avtor), Novinec, Marko (avtor), Lah, Jurij (avtor), Hadži, San (avtor)

Spike trimer plays a key role in SARS-CoV-2 infection and vaccine development. It consists of a globular head and a flexible stalk domain that anchors the protein into the viral membrane. While the head domain has been extensively studied, the properties of the adjoining stalk are poorly understood. Here, we characterize the coiled-coil formation and thermodynamic stability of the stalk domain and its segments. We find that the N-terminal segment of the stalk does not form coiled-coils and remains disordered in solution. The C-terminal stalk segment forms a trimeric coiled-coil in solution, which becomes significantly stabilized in the context of the full-length stalk. Its crystal structure reveals a novel antiparallel tetramer coiled-coil with an unusual combination of a-d and e-a-d hydrophobic core packing. Structural analysis shows that a subset of hydrophobic residues stabilizes different coiled-coil structures: trimer, tetramer, and heterohexamer, underscoring a highly polymorphic nature of the SARS-CoV-2 stalk sequence.

• Jezik: Angleški jezik
• Ključne besede: spike protein, corona virus, coiled-coil, SARS-CoV-2, spike, thermodynamics, X-ray
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 11 str.
• Številčenje: Vol. 36, iss. 3, art. e22199
• PID: 20.500.12556/RUL-136995
• UDK: 577.322
• ISSN pri članku: 0892-6638
• DOI: 10.1096/fj.202101670R
• COBISS.SI-ID: 97817859
• Datum objave v RUL: 27.05.2022

Gradivo je del revije

• Naslov: The FASEB journal
• Skrajšan naslov: FASEB j.
• Založnik: Wiley, Federation of American Societies for Experimental Biology
• ISSN: 0892-6638
• COBISS.SI-ID: 25451520

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: protein spike, korona virus, obvita vijačnica