TBP, PPIA, YWHAZ and EF1A1 are the most stably expressed genes during osteogenic differentiation

Franko, Nina (avtor), Vrščaj, Lucija Ana (avtor), Zore, Taja (avtor), Ostanek, Barbara (avtor), Marc, Janja (avtor), Lojk, Jasna (avtor)

RT-qPCR is the gold standard and the most commonly used method for measuring gene expression. Selection of appropriate reference gene(s) for normalization is a crucial part of RT-qPCR experimental design, which allows accurate quantification and reliability of the results. Because there is no universal reference gene and even commonly used housekeeping genes’ expression can vary under certain conditions, careful selection of an appropriate internal control must be performed for each cell type or tissue and experimental design. The aim of this study was to identify the most stable reference genes during osteogenic differentiation of the human osteosarcoma cell lines MG-63, HOS, and SaOS-2 using the geNorm, NormFinder, and BestKeeper statistical algorithms. Our results show that TBP, PPIA, YWHAZ, and EF1A1 are the most stably expressed genes, while ACTB, and 18S rRNA expressions are most variable. These data provide a basis for future RT-qPCR normalizations when studying gene expression during osteogenic differentiation, for example, in studies of osteoporosis and other bone diseases.

• Jezik: Angleški jezik
• Ključne besede: geNorm, reference gene, NormFinder, BestKeeper, osteogenic differentiation, gene expression, osteosarcoma cell line, RT-qPCR
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 17 str.
• Številčenje: Vol. 23, iss. 8, art. 4257
• PID: 20.500.12556/RUL-136787
• UDK: 575.117:616-006.34
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms23084257
• COBISS.SI-ID: 105244163
• Datum objave v RUL: 20.05.2022

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: referenčni gen, osteogena diferenciacija, celična linija osteosarkoma, gensko izražanje, osteosarkom