Sensing and responding to hypersaline conditions and the HOG signal transduction pathway in fungi isolated from hypersaline environments : Hortaea werneckii and Wallemia ichthyophaga

Plemenitaš, Ana (avtor)

Sensing and responding to changes in NaCl concentration in hypersaline environments is vital for cell survival. In this paper, we identified and characterized key components of the high-osmolarity glycerol (HOG) signal transduction pathway, which is crucial in sensing hypersaline conditions in the extremely halotolerant black yeast Hortaea werneckii and in the obligate halophilic fungus Wallemia ichthyophaga. Both organisms were isolated from solar salterns, their predominating ecological niche. The identified components included homologous proteins of both branches involved in sensing high osmolarity (SHO1 and SLN1) and the homologues of mitogen-activated protein kinase module (MAPKKK Ste11, MAPKK Pbs2, and MAPK Hog1). Functional complementation of the identified gene products in S. cerevisiae mutant strains revealed some of their functions. Structural protein analysis demonstrated important structural differences in the HOG pathway components between halotolerant/halophilic fungi isolated from solar salterns, salt-sensitive S. cerevisiae, the extremely salt-tolerant H. werneckii, and halophilic W. ichthyophaga. Known and novel gene targets of MAP kinase Hog1 were uncovered particularly in halotolerant H. werneckii. Molecular studies of many salt-responsive proteins confirm unique and novel mechanisms of adaptation to changes in salt concentration.

• Jezik: Angleški jezik
• Ključne besede: halotolerant/halophilic fungi, Hortaea werneckii, Wallemia ichthyophaga, HOG, signal transduction pathway
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.02 - Pregledni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 13 str.
• Številčenje: Vol. 7, iss. 11, art. 988
• PID: 20.500.12556/RUL-136365
• UDK: 577
• ISSN pri članku: 2309-608X
• DOI: 10.3390/jof7110988
• COBISS.SI-ID: 86840579
• Datum objave v RUL: 26.04.2022

Gradivo je del revije

• Naslov: Journal of Fungi
• Skrajšan naslov: J. Fungi
• Založnik: MDPI
• ISSN: 2309-608X
• COBISS.SI-ID: 525300761

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 19.11.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: halotolerantne/halofilne glive, Hortaea werneckii, Wallemia ichthyophaga

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0170
• Naslov: Molekulski mehanizmi uravnavanja celičnih procesov v povezavi z nekaterimi boleznimi pri človeku

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Program financ.: Young Researchers