Adhesion and stiffness of detached breast cancer cells in vitro : co-treatment with metformin and 2-deoxy-D-glucose induces changes related to increased metastatic potential

Zemljič Jokhadar, Špela (avtor), Kokot, Gašper (avtor), Pavlin, Mojca (avtor), Derganc, Jure (avtor)

Metastatic cancer cells can overcome detachment-induced cell death and can proliferate in anchorage-independent conditions. A recent study revealed that a co-treatment with two drugs that interfere with cell metabolism, metformin and 2-deoxy-D-glucose, promotes detachment of viable MDA-MB-231 breast cancer cells. In the present study, we analyzed if these detached viable MDA-MB-231 cells also exhibit other features related to cancer metastatic potential, i.e., if they are softer and more prone to adhere to epithelial cells. The cell mechanics of attached cells and floating cells were analyzed by optical tweezers and cell deformability cytometry, respectively. The adhesion was assessed on a confluent monolayer of HUVEC cells, with MDA-MB-231 cells either in static conditions or in a microfluidic flow. Additionally, to test if adhesion was affected by the state of the epithelial glycocalyx, HUVEC cells were treated with neuraminidase and tunicamycin. It was found that the treated MDA-MB-231 cells were more prone to adhere to HUVEC cells and that they were softer than the control, both in the floating state and after re-seeding to a substrate. The changes in the HUVEC glycocalyx, however, did not increase the adhesion potential of MDA-MB-231.

• Jezik: Angleški jezik
• Ključne besede: metastatic potential, adhesion to epithelial cells, optical tweezers, cell deformability cytometry
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 15 str.
• Številčenje: Vol. 10, iss. 9, art. 873
• PID: 20.500.12556/RUL-136166
• UDK: 616-006
• ISSN pri članku: 2079-7737
• DOI: 10.3390/biology10090873
• COBISS.SI-ID: 87303939
• Datum objave v RUL: 14.04.2022

Gradivo je del revije

• Naslov: Biology
• Skrajšan naslov: Biology
• Založnik: MDPI
• ISSN: 2079-7737
• COBISS.SI-ID: 523004441

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 04.09.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: metastatski potencial, adhezija na epitelijske celice, optične pincete

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0055
• Naslov: Biofizika polimerov, membran, gelov, koloidov in celic

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-6794
• Naslov: Celična energijska presnova kot tarča za zdravljenje raka – genski in farmakološki pristop