High genetic diversity and low population differentiation in wild hop (Humulus lupulus L.) from Croatia

Grdiša, Martina (avtor), Šatović, Zlatko (avtor), Liber, Zlatko (avtor), Jakše, Jernej (avtor), Varga, Filip (avtor), Erhatić, Renata (avtor), Srečec, Siniša (avtor)

Hop (Humulus lupulus L.) is used in the brewing industry as a source of compounds responsible for the bitterness, aroma, and preservative properties of beer. In this study, we used microsatellite markers to investigate genetic diversity and genetic differentiation of wild hop populations sampled in the northwestern part of Croatia. Analysis of 12 microsatellite loci revealed high diversity and weak population differentiation among wild hop populations. A total of 152 alleles were determined with an average of 12.67 alleles per locus. Observed heterozygosity ranged from 0.689 to 0.839 (average 0.767) and expected heterozygosity ranged from 0.725 to 0.789 (average 0.760). A total of 38 private alleles were detected. The data suggest that H. lupulus populations are not affected by recent bottlenecks. The degree of genetic differentiation among populations was low and not significant for most pairwise F$_{ST}$ values, except for the pair of geographically most distant populations. The results did not indicate the existence of genetic structure among the sampled populations. The high genetic diversity and low differentiation among populations, combined with the absence of isolation by distance, indicate the existence of substantial gene flow among wild hop populations. Therefore, extensive sampling per population is clearly required to assess the genetic diversity of hop populations. Sampling strategies involving sampling across a large number of localities represented by only a few samples could lead to erroneous conclusions.

• Jezik: Angleški jezik
• Ključne besede: genetic diversity and structure, genetic resources, Humulus lupulus L., SSR, wild germplasm
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 14 str.
• Številčenje: Vol. 11, iss. 14, art. 6484
• PID: 20.500.12556/RUL-136011
• UDK: 631
• ISSN pri članku: 2076-3417
• DOI: 10.3390/app11146484
• COBISS.SI-ID: 74705923
• Datum objave v RUL: 06.04.2022

Gradivo je del revije

• Naslov: Applied sciences
• Skrajšan naslov: Appl. sci.
• Založnik: MDPI
• ISSN: 2076-3417
• COBISS.SI-ID: 522979353

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 14.07.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: genetska raznolikost, genetski viri, Humulus lupulus, divji hmelj, Hrvaška