Identification and validation of new cancer stem cell-related genes and their regulatory microRNAs in colorectal cancerogenesis

Urh, Kristian (avtor), Žlajpah, Margareta (avtor), Zidar, Nina (avtor), Boštjančič, Emanuela (avtor)

Significant progress has been made in the last decade in our understanding of the pathogenetic mechanisms of colorectal cancer (CRC). Cancer stem cells (CSC) have gained much attention and are now believed to play a crucial role in the pathogenesis of various cancers, including CRC. In the current study, we validated gene expression of four genes related to CSC, L1TD1, SLITRK6, ST6GALNAC1 and TCEA3, identified in a previous bioinformatics analysis. Using bioinformatics, potential miRNA-target gene correlations were prioritized. In total, 70 formalin-fixed paraffin-embedded biopsy samples from 47 patients with adenoma, adenoma with early carcinoma and CRC without and with lymph node metastases were included. The expression of selected genes and microRNAs (miRNAs) was evaluated using quantitative PCR. Differential expression of all investigated genes and four of six prioritized miRNAs (hsa-miR-199a-3p, hsa-miR-335-5p, hsa-miR-425-5p, hsa-miR-1225-3p, hsa-miR-1233-3p and hsa-miR-1303) was found in at least one group of CRC cancerogenesis. L1TD1, SLITRK6, miR-1233-3p and miR-1225-3p were correlated to the level of malignancy. A negative correlation between miR-199a-3p and its predicted target SLITRK6 was observed, showing potential for further experimental validation in CRC. Our results provide further evidence that CSC-related genes and their regulatory miRNAs are involved in CRC development and progression and suggest that some them, particularly miR-199a-3p and its SLITRK6 target gene, are promising for further validation in CRC.

• Jezik: Angleški jezik
• Ključne besede: colorectal cancer, differentially expressed genes, cancer stem cells, qPCR
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 20 str.
• Številčenje: Vol. 9, iss. 2, art. 179
• PID: 20.500.12556/RUL-135037
• UDK: 616-006
• ISSN pri članku: 2227-9059
• DOI: 10.3390/biomedicines9020179
• COBISS.SI-ID: 53489155
• Datum objave v RUL: 18.02.2022

Gradivo je del revije

• Naslov: Biomedicines
• Skrajšan naslov: Biomedicines
• Založnik: MDPI
• ISSN: 2227-9059
• COBISS.SI-ID: 523006745

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 11.02.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kolorektalni rak, različno izraženi geni, matične celice raka