Comparison of Campylobacter jejuni slaughterhouse and surface-water isolates indicates better adaptation of slaughterhouse isolates to the chicken host environment

Šimunović, Katarina (avtor), Zajkoska, Sandra (avtor), Bezek, Katja (avtor), Klančnik, Anja (avtor), Barlič-Maganja, Darja (avtor), Smole Možina, Sonja (avtor)

Campylobacter jejuni is an emerging food-borne pathogen that poses a high risk to human health. Knowledge of the strain source can contribute significantly to an understanding of this pathogen, and can lead to improved control measures in the food-processing industry. In this study, slaughterhouse and surface-water isolates of C. jejuni were characterized and compared in terms of their antimicrobial resistance profiles and adhesion to stainless steel and chicken skin. Resistance of C. jejuni biofilm cells to benzalkonium chloride and Satureja montana ethanolic extract was also tested. The data show that the slaughterhouse isolates are more resistant to ciprofloxacin, and adhere better to stainless steel at 42 °C, and at 37 °C in 50% chicken juice. Additionally, biofilm cells of the isolate with the greatest adhesion potential (C. jejuni S6) were harvested and tested for resistance to S. montana ethanolic extract, benzalkonium chloride, and erythromycin; and for efflux-pump activity, as compared to their planktonic cells. The biofilm cells showed increased resistance to both S. montana ethanolic extract and erythromycin, and increased efflux-pump activity. These data indicate adaptation of C. jejuni slaughterhouse isolates to the chicken host, as well as increased biofilm cell resistance due to increased efflux-pump activity.

• Jezik: Angleški jezik
• Ključne besede: Campylobacter jejuni, slaughterhouse isolates, surface-water isolates, biofilm resistance, efflux pump activity
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 15 str.
• Številčenje: Vol. 8, iss. 11, art. 1693
• PID: 20.500.12556/RUL-134698
• UDK: 579.22/.26:547.9:615.281
• ISSN pri članku: 2076-2607
• DOI: 10.3390/microorganisms8111693
• COBISS.SI-ID: 35369987
• Datum objave v RUL: 26.01.2022

Gradivo je del revije

• Naslov: Microorganisms
• Skrajšan naslov: Microorganisms
• Založnik: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.11.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: patogeni mikroorganizmi, Campylobacter jejuni, biofilmi, klavniški sevi, protimikrobno delovanje, rastlinski izvlečki, kraški šetraj, Satureja montana, etanolni izvlečki, benzalkonijev klorid, izlivne črpalke