Vrednotenje učinkovitosti ekstrakcije pigmentov iz liofiliziranih živil rastlinskega izvora z bioobnovljivim topilom CyreneTM

Zupanc, Sendy (avtor), Cigić, Blaž (mentor), Polak, Tomaž (komentor)

Magistrska naloga obravnava karakterizacijo bioobnovljivega topila CyreneTM in njegovo uporabo za namen ekstrakcije pigmentov iz liofiliziranih vzorcev rastlinskega izvora. Ugotovili smo, da CyreneTM močno absorbira v UV delu spektra, medtem ko je absorbanca nad 430 nm zanemarljiva. Vodne raztopine CyreneTM so kisle (pH = 3,8). CyreneTM je reaktiven v testih za določevanje antioksidativnega potenciala. V Folin Ciocalteu testu smo določili za CyreneTM 132 TE/L 100 % CyreneTM, v ABTS testu 20,7 TE/L 100 % CyreneTM in v DPPH testu 13,2 TE/L 100 % CyreneTM. Z vodnimi raztopinami CyreneTM smo učinkovito ekstrahirali pigmente iz liofilizirane pšenične trave, koruznega glutena, korenčka, rdeče pese, aronije in borovnice. Nepolarne karotene iz korenčka smo najučinkoviteje ekstrahirali s 100 % CyreneTM, polarnejše ksantofile iz skupine karotenoidov, s katerimi je bogat koruzni gluten, pa z 80 % CyreneTM. Klorofili iz pšenične trave so se najbolj učinkovito ekstrahirali s 95 % CyreneTM, medtem ko je bila ekstrakcija relativno polarnih antocianinov iz aronije in borovnice ter betalainov iz rdeče pese najučinkovitejša s 40 % do 60 % CyreneTM. Razmerje med liofilizatom in topilom v razponu od 1:10 do 1:100 je imelo relativno majhen vpliv na učikovitost ekstrakcije. Primerjava učinkovitosti ekstrakcije z vodnimi raztopinami CyreneTM in tradicionalno uporabljenimi topili je razkrila, da se betalaini najučinkoviteje ekstrahirajo z raztopino CyreneTM, medtem ko je ekstrakcija ostalih pigmentov slabša v primerjavi s klasičnimi topili.

• Jezik: Slovenski jezik
• Ključne besede: rastlinski pigmenti, ekstrakcija, topilo, CyreneTM, UV-VIS spektrofotometrija, DPPH, ABTS, Folin-Ciocalteu, antioksidanti
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: BF - Biotehniška fakulteta
• Kraj izida: Ljubljana
• Založnik: [S. Zupanc]
• Leto izida: 2022
• PID: 20.500.12556/RUL-134629
• UDK: 547.97:66.061.18:633.86
• COBISS.SI-ID: 97027331
• Datum objave v RUL: 22.01.2022

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Evaluation of the efficiency of extraction of pigments from lyophilized foods of plant origin with the bio-renewable solvent CyreneTM

Izvleček

The master's thesis aims to characterize the sustainable solvent CyreneTM and its application for the extraction of pigments from lyophilized samples of plant origin. We found that CyreneTM strongly absorbs in the UV part of the spectrum, while is the absorbance above 430 nm negligible. CyreneTM aqueous solutions are acidic (pH = 3.8). CyreneTM interfers in tests for the analysis of antioxidant potential. In the Folin Ciocalteu test, CyreneTM gives 132 TE/L 100 % CyreneTM, 20.7 TE/L 100 % CyreneTM in the ABTS test and 13.2 TE/L 100 % CyreneTM in the DPPH test. Pigments from lyophilized wheat grass, corn gluten, carrot, beetroot, chokeberry and blueberry were effectively extracted with aqueous CyreneTM solutions. Nonpolar carotenes from carrot were the most efficiently extracted with 100 % CyreneTM, and more polar xanthophylls of the carotenoid group, from corn gluten, with 80 % CyreneTM. Chlorophylls from wheat grass were most efficiently extracted with 95% CyreneTM, while extraction of relatively polar anthocyanins from chokeberry and blueberry, and betalaine from beetroot was the most effective with 40% to 60% CyreneTM. The ratio of lyophilisate to solvent in the range of 1:10 to 1: 100 had a relatively small effect on the extraction efficiency. A comparison of the extraction efficiency with aqueous CyreneTM solutions and traditionally used solvents revealed that betalains are the most efficiently extracted with CyreneTM solution, while the extraction of other pigments is poorer as compared to conventional solvents.

• Ključne besede: plant pigments, extraction, solvent, CyreneTM, UV-VIS spectroscopy, DPPH, ABTS, Folin-Ciocalteu, antioxidants